Monoclonal Anti-CD8 antibody produced in mouse

Monoclonal Anti-Human CD8 (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from BALB/c mice immunized with human thymocytes followed by peripheral blood T cells. CD8 (cluster of differentiation 8), is a dimeric protein, encoded by the CD8A gene mapped to human chromosome 2p12.

Recognizes the CD8 human T cytotoxic/suppressor lymphocyte cell surface glycoprotein. Approximately 90% of thymocytes are stained in suspension. In thymus sections, both cortical and medullary thymocytes are stained. A subset of NK cells express this antigen weakly. The epitope recognized by this clone is sensitive to routine formalin fixation and paraffin embedding.
3rd Workshop: code no. 567

human thymocytes followed by peripheral blood T cells.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Immunofluorescence (1 paper)

Monoclonal Anti-CD8 antibody produced in mouse is has been used for the following:

• enumeration of total T cytotoxic/suppressor lymphocytes in bone marrow, blood and other body fluids
• identification and localization of T cytotoxic/ suppressor lymphocytes in lymphoid and other tissues
• analysis of cell mediated cytotoxicity
• studies of immunoregulation in health and disease
• investigation of natural killer (NK) cells
• complement mediated cytolysis of CD8 expressing cells

Monoclonal Anti-CD8 antibody produced in mouse is suitable for flow cytometry at a dilution of 5μL using 1 × 106 cells.

CD8 might serve as a marker for neoplastic diseases and acute myeloid leukemia.

CD8a (Cluster of Differentiation 8a) is a human gene. CD8 binding to class I MHC and the co-receptor′s during the process of cytotoxic T-cell activation. T cells expresses T cell receptor (TCR) complexes which lacks CD3 delta, exhibit severely impaired positive selection and TCR-mediated activation of CD8 single-positive T cells. CD3 delta couples with TCR. The CD3 associated with CD8 is required for effective activation and positive selection of CD8(+) T cells. CD8 expression is a recurrent rare phenomenon in patients with chronic lymphocytic leukemia (CLL).

CD8 is a two chain complex (α/α, α/β) receptor for Class I MHC molecules. The receptor′s cytoplasmic portion is associated with p56^lck protein tyrosine kinase which phosphorylates nearby proteins. The antigen is strongly expressed on approximately one third of mature T cells (cytotoxic/suppressor T cells).

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

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