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C7352

Sigma-Aldrich

L-半胱氨酸

from non-animal source, BioReagent, suitable for cell culture, ≥98%

别名:

(R)-2-氨基-3-巯基丙酸

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About This Item

线性分子式:
HSCH2CH(NH2)CO2H
CAS号:
52-90-4
分子量:
121.16
Beilstein:
1721408
EC 号:
200-158-2
MDL编号:
MFCD00064306
UNSPSC代码:
12352209
eCl@ss:
32160406
PubChem化学物质编号:
24892959
NACRES:
NA.75
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生物来源

non-animal source

质量水平

200

产品线

BioReagent

方案

≥98%

表单

crystalline powder

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

颜色

white

mp

240 °C (dec.) (lit.)

溶解性

H2O: 25 mg/mL

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SAB1300288HPA031151SAE0093
Sigma-Aldrich

44498

β-1,4-Galactosyl Transferase I human, recombinant from Saccharomyces cerevisiae

Anti-GCNT1 (center) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

SAB1300288

Anti-GCNT1 (center) antibody produced in rabbit

Anti-GCNT1 antibody produced in rabbit Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

HPA031151

Anti-GCNT1 antibody produced in rabbit

Beta-1,4-galactosyltransferase 1 B4GALT1 human recombinant, expressed in HEK 293 cells, 2000 units/mg protein

SAE0093

Beta-1,4-galactosyltransferase 1

Gene Information

human ... GCNT1(2650)

Gene Information

human ... GCNT1(2650)

Gene Information

human ... GCNT1(2650)

Gene Information

-

recombinant

expressed in Saccharomyces cerevisiae

recombinant

-

recombinant

-

recombinant

expressed in HEK 293 cells

concentration

≥0.2 unit/mL, 0.5-4 mg/mL protein

concentration

-

concentration

-

concentration

-

UniProt accession no.

Q02742

UniProt accession no.

Q02742

UniProt accession no.

Q02742

UniProt accession no.

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

impurities

≤0.1% β-galactosidase

impurities

-

impurities

-

impurities

-

一般描述

L-半胱氨酸是一种含硫的蛋白质原性氨基酸，根据遗传密码与蛋白质结合。[1]硫分子侧链参与细胞内的各种氧化/还原反应。侧链参与β键的形成，调节蛋白质二级和三元结构。L-半胱氨酸是无机硫酸盐辅酶A的重要前体。[1]半胱氨酸是细胞内谷胱甘肽（一种重要的抗氧化剂）的形成必不可少且有限制作用。哺乳动物肝脏可合成L-半胱氨酸，这是一种半非必需氨基酸。L-半胱氨酸能防止神经细胞氧化，有助于维持结缔组织的稳定性和硬度。L-半胱氨酸可加速机体的愈合过程并减少炎症反应。L-半胱氨酸可部分保护睾丸抵御丙烯酰胺的毒性。[1]L-半胱氨酸可以防止DNA在低温储存过程中的损伤，保持精子质量。[2]
L-半胱氨酸是一种蛋白质原性氨基酸，根据遗传密码与蛋白质结合。 硫分子侧链参与细胞内的各种氧化/还原反应。 侧链参与β键的形成，调节蛋白质二级和三元结构。半胱氨酸是细胞内谷胱甘肽（一种重要的抗氧化剂）的形成必不可少且有限制作用。

应用

L-半胱氨酸已可用于：
  • 作为蓝氏贾第鞭毛虫培养用胰蛋白酶-酵母提取物-铁血清(TYI-S-33)培养基的补充物，[3]通过Dulbecco′s改良Eagles培养基(DMEM)配制酶溶液，建立海马培养模型[4]
  • 研究其对丙烯酰胺致睾丸毒性的保护作用[1]
  • 制备精子超低温保存的三羧酸冻存液[2]
  • 作为木瓜蛋白酶消化液的添加剂[5]

生化/生理作用

NMDA 谷氨酸受体激动剂。

其他说明

L-半胱氨酸用来补充细胞培养基。 该产品是一种非动物源氨基酸，适用于无血清培养基配方的生物制造应用。

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    0000444033
    0000444032
    0000444028
    0000444028
    0000444032

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    Preparation of rat brain aggregate cultures for neuron and glia development studies
    Koito H and Li J
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    An image-based assay for high throughput screening of Giardia lamblia
    Gut J, et al.
    Journal of Microbiological Methods, 84(3), 398-405 (2011)
    Effect of L-Cysteine on post thaw quality of Nili Ravi buffalo (Bubalus bubalis) bull spermatozoa cryopreserved in tris citric acid extender
    Wadood F, et al.
    The Journal of Animal and plant sciences, 25, 301-303 (2015)
    L-Cysteine Partially Protects Against Acrylamide-Induced Testicular Toxicity.
    Kacar S, et al.
    Balkan medical journal, 35(4), 311-311 (2018)
    Fibroblast growth factor 23 signaling in hippocampal cells: impact on neuronal morphology and synaptic density
    Hensel N, et al.
    Journal of Neurochemistry, 137(5), 756-769 (2016)
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