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Merck
CN

C5467

SAFC

EX-CELL ® ACF CHO 培养基

Animal-component free, with HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Pharma Manufacturing

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别名:
CHO 培养基
MDL编号:
UNSPSC代码:
12352207
NACRES:
NA.75

质量水平

描述

for research or for further manufacturing use

无菌性

sterile-filtered

形式

liquid

质量

Drug/Device Master File available

技术

cell culture | mammalian: suitable

组分

HEPES: yes
NaHCO3: yes
phenol red: no
L-glutamine: no

运输

ambient

储存温度

2-8°C

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应用

用于优化中国仓鼠卵巢 (CHO) 细胞中细胞生长和蛋白表达的无动物成分培养基。

特点和优势

为满足生物技术和疫苗制造的需要而开发,该培养基支持悬浮培养物中快速的初始细胞生长和高水平的蛋白表达。它也支持高细胞密度和维持这些密度的活细胞为长期导致生产力提高。在分批培养系统中已经实现了每毫升超过 8×106 个细胞的细胞密度。已观察到每个细胞的蛋白质生产率增加高达 500%。

其他说明

专利配方,含有无机盐、HEPES 和碳酸氢钠缓冲液、必需和非必需氨基酸、维生素、重组人胰岛素、植物水解物、其他有机化合物、微量元素和表面活性剂。
不含抗生素、抗真菌药、 L -谷氨酰胺或转铁蛋白。不含动物源性蛋白或其他成分。

重悬

使用前,每升培养基中无菌加入 20-40 ml 200 mM L-谷氨酰胺溶液。

法律信息

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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商品

Cell culture protocol for passaging and splitting suspension cell lines

用于传代和分装悬浮细胞系的细胞培养方案

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.

In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.

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