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一般描述
分子量： 280 kDa
等电点： 5.5
消光系数： E1%= 18.0（280 nm）
来自 Electrophorus electricus 的乙酰胆碱酯酶是由 4 个相等的亚基组成的四聚体，每个亚基 70kDa。每个子单元包含一个活性位点。该酶是含有己糖胺的糖蛋白。
等电点： 5.5
消光系数： E1%= 18.0（280 nm）
来自 Electrophorus electricus 的乙酰胆碱酯酶是由 4 个相等的亚基组成的四聚体，每个亚基 70kDa。每个子单元包含一个活性位点。该酶是含有己糖胺的糖蛋白。
应用
来自sigma的酶已被用作评估阿斯巴甜代谢物对海马乙酰胆碱酯酶活性影响的参考。该酶还用于快速检测乙酰硫代胆碱氯化物的固定化研究中。
生化/生理作用
体内乙酰胆碱的主要降解酶。 将乙酰胆碱 + H2O 转化为胆碱+乙酸。
单位定义
一单位在37°C、pH 8.0条件下每分钟可将1.0 μ摩尔的乙酰胆碱水解成胆碱和醋酸盐 。
外形
含 Tris 缓冲盐的冻干粉
制备说明
该酶可在20 mM Tris-HCl, pH 7.5中以1 mg/mL的浓度溶解并产生清亮的溶液。
分析说明
使用乙酰胆碱作为底物而获得的活性是丁基胆碱通过使用来自电鳗的乙酰胆碱酯酶而获得活性的30-100倍。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
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