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类型
Type VI-S
表单
lyophilized powder
比活
200-1,000 units/mg protein
分子量
280 kDa
组成
Protein, ≥45% biuret
溶解性
20 mM Tris HCl buffer, pH 7.5: soluble 1.0 mg/mL, clear(lit.)
应用
diagnostic assay manufacturing
储存温度
−20°C
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此商品 | 130672 | 774324 | 633259 |
|---|---|---|---|
| form solid | form solid | form solid | form solid |
| quality LiChropur™ | quality - | quality - | quality - |
| reaction suitability reagent type: derivatization reagent | reaction suitability - | reaction suitability - | reaction suitability - |
| technique(s) LC/MS: suitable | technique(s) - | technique(s) - | technique(s) protein expression: suitable |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level - |
一般描述
分子量： 280 kDa
等电点： 5.5
消光系数： E1%= 18.0（280 nm）
来自 Electrophorus electricus 的乙酰胆碱酯酶是由 4 个相等的亚基组成的四聚体，每个亚基 70kDa。每个子单元包含一个活性位点。该酶是含有己糖胺的糖蛋白。
等电点： 5.5
消光系数： E1%= 18.0（280 nm）
来自 Electrophorus electricus 的乙酰胆碱酯酶是由 4 个相等的亚基组成的四聚体，每个亚基 70kDa。每个子单元包含一个活性位点。该酶是含有己糖胺的糖蛋白。
生化/生理作用
体内乙酰胆碱的主要降解酶。 将乙酰胆碱 + H2O 转化为胆碱+乙酸。
单位定义
一单位在37°C、pH 8.0条件下每分钟可将1.0 μ摩尔的乙酰胆碱水解成胆碱和醋酸盐 。
外形
含 Tris 缓冲盐的冻干粉
制备说明
该酶可在20 mM Tris-HCl, pH 7.5中以1 mg/mL的浓度溶解并产生清亮的溶液。
分析说明
使用乙酰胆碱作为底物而获得的活性是丁基胆碱通过使用来自电鳗的乙酰胆碱酯酶而获得活性的30-100倍。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Jingming Gong et al.
Biosensors & bioelectronics, 24(7), 2285-2288 (2008-12-30)
We developed a simple strategy for designing a highly sensitive electrochemical biosensor for organophosphate pesticides (OPs) based on acetylcholinesterase (AChE) immobilized onto Au nanoparticles-polypyrrole nanowires composite film modifid glassy carbon electrode (labeled as AChE-Au-PPy/GCE). Where, the generated Au nanoparticles (AuNPs)
Gabriela Villalta et al.
Plants (Basel, Switzerland), 10(6) (2021-07-03)
The essential oil (EO) of Salvia leucantha Cav. was isolated by steam distillation of the aerial parts collected in the South of Ecuador. Its physical properties were evaluated and the chemical composition of the oil was determined by GC-MS and
Makar Makarian et al.
Journal of molecular structure, 1247 (2022-03-01)
In an effort to develop new therapeutic agents to treat Alzheimer's disease, a series of donepezil-based analogs were designed, synthesized using an environmentally friendly route, and biologically evaluated for their inhibitory activity against electric eel acetylcholinesterase (AChE) enzyme. In vitro
Dan Du et al.
Analytical and bioanalytical chemistry, 387(3), 1059-1065 (2006-12-23)
A simple method has been devised for immobilization of acetylcholinesterase (AChE)--covalent bonding to a multiwall carbon nanotube (MWNT)--cross-linked chitosan composite (CMC)-and a sensitive amperometric sensor for rapid detection of acetylthiocholine (ATCl) has been based on this. Fourier-transform infrared spectroscopy proved
Acetylcholinesterase, I. Large-scale purification, homogeneity, and amino Acid analysis.
W Leuzinger et al.
Proceedings of the National Academy of Sciences of the United States of America, 57(2), 446-451 (1967-02-01)
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