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Merck
CN

C2999

Sigma-Aldrich

CryoStor® 细胞冻存培养基

CS5

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别名:
cell freezing medium
UNSPSC代码:
12352207
NACRES:
NA.75

质量水平

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable
cryopreservation: suitable

运输

ambient

储存温度

2-8°C

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一般描述

CryoStor CS2、CS5 和 CS10 系列保存溶液代表了下一代冷冻保存培养基。CryoStor 培养基设计用于在超低温环境(-80 至 -196°C)内制备和保存细胞,在冷冻、储存和解冻过程中为细胞和组织提供安全的保护环境。通过调节冻存过程中的细胞生化反应,这些培养基提供了增强的细胞活力和功能性,同时不需要添加血清、蛋白质或高水平的细胞毒性试剂。

Cryostor CS2、CS5 和 CS10 是一系列细胞特异性的、优化的冻存培养基,其独特配方可解决细胞在冻存过程中的分子生物学问题,从而直接降低冻存诱导的迟发性细胞死亡的水平,提高解冻后细胞的活力和功能。

这些培养基被推荐用于冻存干细胞、肝细胞、组织样本和其他非常敏感的细胞类型。

应用

CryoStor 是一系列细胞特异性的、优化的冻存培养基,具有独特的配方,可在冻存过程中解决细胞的分子生物学问题,从而直接降低冻存诱导的迟发性细胞死亡,并提高解冻后细胞的活力和功能。
Cryostor CS5含5%二甲基亚砜(DMSO)。推荐大多数细胞类型选择其用于超低温保藏。

Cryostor® 冷冻保存视频操作流程

其他说明

配方含有5% DMSO。

法律信息

CryoStor is a registered trademark of BioLife Solutions, Inc.

象形图

Corrosion

警示用语:

Warning

危险声明

预防措施声明

危险分类

Met. Corr. 1

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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Hui Li et al.
Journal of virology (2021-03-05)
Previously, we showed that substitution of HIV-1 Env residue 375-Ser by bulky aromatic residues enhances binding to rhesus CD4 and enables primary HIV-1 Envs to support efficient replication as simian-human immunodeficiency virus (SHIV) chimeras in rhesus macaques (RMs). Here, we
Dominic M Clarke et al.
Cytotherapy, 11(4), 472-479 (2009-06-06)
Peripheral blood stem cells (PBSC) have become the preferred stem cell source for autologous hematopoietic transplantation. A critical aspect of this treatment modality is cryopreservation of the stem cell products, which permits temporal separation of the PBSC mobilization/collection phase from
Ryan S Roark et al.
Science (New York, N.Y.), 371(6525) (2020-11-21)
Neutralizing antibodies elicited by HIV-1 coevolve with viral envelope proteins (Env) in distinctive patterns, in some cases acquiring substantial breadth. We report that primary HIV-1 envelope proteins-when expressed by simian-human immunodeficiency viruses in rhesus macaques-elicited patterns of Env-antibody coevolution very
J Stylianou et al.
Cytotherapy, 8(1), 57-61 (2006-04-22)
Hematopoietic stem cells (HSC) have traditionally been frozen using the cryoprotectant DMSO in dextran-40, saline or albumin. However, the process of freezing and thawing results in loss of HSC numbers and/or function. This study investigated the use of CryoStor for
Haichen Niu et al.
Brain pathology (Zurich, Switzerland), 30(6), 1102-1118 (2020-07-18)
Olfactory dysfunction is one of the early symptoms seen in Parkinson's disease (PD). However, the mechanisms underlying olfactory pathology that impacts PD disease progression and post-mortem appearance of alpha-Synuclein (α-Syn) inclusions in and beyond olfactory bulb in PD remain unclear.

商品

用于细胞系冷冻保存的良好细胞库操作实践概述。

Overview of good cell banking practices for cell line cryopreservation purposes.

实验方案

Cryopreservation affects post-thaw recovery, viability, and functionality. Stress during freezing and suboptimal media lead to cell death.

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