biological source
Electrophorus electricus
Quality Level
type
Type V-S
form
lyophilized powder
specific activity
≥1,000 units/mg protein
mol wt
280 kDa
composition
Protein, ≥45% biuret
pI
~5.3
solubility
Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)
storage temp.
−20°C
General description
分子量: 280 kDa
等电点: 5.5
消光系数: E1%= 18.0(280 nm)
来自 Electrophorus electricus 的乙酰胆碱酯酶是由 4 个相等的亚基组成的四聚体,每个亚基 70kDa。每个子单元包含一个活性位点。该酶是含有己糖胺的糖蛋白。
等电点: 5.5
消光系数: E1%= 18.0(280 nm)
来自 Electrophorus electricus 的乙酰胆碱酯酶是由 4 个相等的亚基组成的四聚体,每个亚基 70kDa。每个子单元包含一个活性位点。该酶是含有己糖胺的糖蛋白。
Application
电鳗乙酰胆碱酯酶已用于:
- 乙酰胆碱酯酶活性检测
- 研究其对爪蟾胚胎海马体细胞的影响
- 研究乙酰胆碱抑制剂对突触发育和可塑性的影响
Biochem/physiol Actions
体内乙酰胆碱的主要降解酶。 将乙酰胆碱 + H2O 转化为胆碱+乙酸。
乙酰胆碱酯酶(AChE)的活性表现于其可降解神经肌肉接头和胆碱能神经元突触间隙处的乙酰胆碱。该酶参与一氧化氮信号通路。
Physical form
含 Tris 缓冲盐的冻干粉
Analysis Note
使用乙酰胆碱作为底物获得的活性是用丁基胆碱获得的活性的 30-100 倍,使用来自电鳗的乙酰胆碱酯酶。
Other Notes
一个单元将在 pH8.0,37℃下,每分钟将 1.0 μmole 乙酰胆碱水解成胆碱和乙酸盐。
Disclaimer
对于稀释溶液(<1 mg/ml),加入 1mg/ml BSA 以稳定。在稀释的中性磷酸盐缓冲液中含有至少 1mg 蛋白质/ml 的溶液在冷藏至少 6 个月后是稳定的。
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
动植物源性产品
此项目有
Joana R Almeida et al.
Biomolecules, 10(8) (2020-08-06)
The development of alternative ecological and effective antifouling technologies is still challenging. Synthesis of nature-inspired compounds has been exploited, given the potential to assure commercial supplies of potential ecofriendly antifouling agents. In this direction, the antifouling activity of a series
Silvia Olivera et al.
Molecular and cellular neurosciences, 23(1), 96-106 (2003-06-12)
Here we show that chronic application of low concentrations (0.01-0.05 U/ml) or a single application of 1-5 U/ml acetylcholinesterase (AChE) promotes the extension of neuronal processes, synapse formation, and alpha-amino-3-hydroxy-5-methylisoxazolepropionate receptor (AMPAR) surface expression in both embryonic and postnatal hippocampal
Daniela Pereira et al.
Marine drugs, 19(1) (2020-12-31)
Over the last decades, antifouling coatings containing biocidal compounds as active ingredients were used to prevent biofouling, and eco-friendly alternatives are needed. Previous research from our group showed that polymethoxylated chalcones and glycosylated flavones obtained by synthesis displayed antifouling activity
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| C2888-5KU | 04061833492420 |
| C2888-500UN | 04061835543267 |
| C2888-1KU | 04061835555901 |