推荐产品
生物来源
sheep
质量水平
偶联物
CY3 conjugate
抗体形式
affinity isolated antibody
抗体产品类型
secondary antibodies
克隆
polyclonal
表单
buffered aqueous solution
种属反应性
rabbit
技术
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
一般描述
免疫球蛋白G(IgG)的单体结构由两条相同的重链和两条相同的轻链构成,其分子量分别为50kDa和25kDa。该抗体的一级结构还包含连接两条重链、连接重链和轻链,并位于链内的二硫键。IgG 可进一步分为四种亚型,即IgG1、IgG2、IgG3 和 IgG4,而这四种亚型所含的不同重链分别称为 γ1、 γ2、 γ3 和 γ4。使用木瓜蛋白酶的有限消化将抗体切割成三个片段,其中两个片段相同,并具有抗原结合活性(Fab片段)。
绵羊体内产生的抗兔 IgG(全分子)、F(ab′)2 片段 – Cy3 抗体可与所有兔 Ig 结合,在试图避免由于 Fc 受体的存在而导致背景染色时有用。
应用
绵羊抗兔IgG(全分子)、F(ab′)2片段-Cy3抗体可用于1:100替代的免疫组化和免疫细胞化学。
生化/生理作用
IgG抗体亚型是免疫系统中最丰富的血清免疫球蛋白。它由B细胞分泌,存在于血液和细胞外液中,可防止细菌、真菌和病毒引起的感染。母体IgG可通过胎盘转移至胎儿,胎盘对新生儿免疫防御感染至关重要。Cy3与抗兔IgG(全分子),F(ab′)2片断抗体的偶联可通过荧光显微镜观察蛋白质。
外形
0.01 M 磷酸盐缓冲液 (pH 7.4) 中的溶液,含 1% 牛血清白蛋白和 15 mM 叠氮化钠
法律信息
Cy由Amersham Biosciences Limited许可分销。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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储存分类代码
10 - Combustible liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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