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一般描述
使用克隆环，可以从含有许多克隆的培养板中分离并挑选出转染细胞的单个菌落。分离的克隆与周围细胞隔离，可解离并传代，或者用50-100μL生长培养基脉冲，以便分析分泌物。无菌克隆环的一端涂有润滑脂，以便密封克隆环与培养板的接触面。随后，在克隆环内将细胞解离和悬浮，并作为纯菌落转移到新的容器中。
使用电子束照射将这些克隆环灭菌。 以合适的剂量水平灭菌后，玻璃克隆环将呈棕色。
使用电子束照射将这些克隆环灭菌。 以合适的剂量水平灭菌后，玻璃克隆环将呈棕色。
包装
包装在60 mm培养皿中，其中一端涂有硅脂
Tamara D Kolokoltsova et al.
Human cell, 30(4), 249-257 (2017-06-15)
In the present study, we describe a new method of isolation and culture of human villous and extravillous trophoblasts from term placenta. The cultivation of trypsinized placental villous tissue explants, followed by the isolation of cells from outgrowth islets allows
D Geerts et al.
The Journal of cell biology, 147(2), 417-434 (1999-10-20)
Hemidesmosomes are stable adhesion complexes in basal epithelial cells that provide a link between the intermediate filament network and the extracellular matrix. We have investigated the recruitment of plectin into hemidesmosomes by the alpha6beta4 integrin and have shown that the
Maria Chiara Lionetti et al.
Biophysical journal, 118(9), 2319-2332 (2020-04-23)
The nuclear morphology of eukaryotic cells is determined by the interplay between the lamina forming the nuclear skeleton, the chromatin inside the nucleus, and the coupling with the cytoskeleton. Nuclear alterations are often associated with pathological conditions as in Hutchinson-Gilford
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