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安全信息

A8592

Sigma-Aldrich

单克隆抗-FLAG® M2-过氧化物酶（HRP） 小鼠抗

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

别名:

单克隆抗-FLAG® M2 小鼠抗, 抗 ddddk, 抗 dykddddk

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About This Item

UNSPSC代码:
41106514
NACRES:
NA.32

生物来源

mouse

质量水平

200

偶联物

peroxidase conjugate

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

M2, monoclonal

形式

buffered aqueous glycerol solution

种属反应性

all

浓度

~1 mg/mL

技术

indirect ELISA: 1:20,000

同位素/亚型

IgG1

免疫原序列

DYKDDDDK

运输

wet ice

储存温度

−20°C

基因信息

human ... GPX1(2876)

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相关类别

一般描述

单克隆抗-FLAG M2-过氧化物酶是一种共价偶联至辣根过氧化物酶（HRP）的小鼠IgG抗体。该抗体可与FLAG融合蛋白结合，并在N末端、Met-N末端、C末端和内部FLAG肽识别FLAG表位。

应用

用于免疫细胞化学及蛋白免疫印迹的建议稀释比是1:100至1:1000
用于ELISA的建议稀释比是1:20,000

请访问我们的FLAG®应用门户网站，了解更多产品详情。
用于通过免疫细胞化学、ELIS或Western blotting的简单一步法检测。 特别适用于检测小鼠二抗体可能会引起交叉反应的鼠宿主中表达的FLAG融合蛋白。

外形

在含有50％甘油以及防腐剂和稳定剂的磷酸盐缓冲盐溶液中

制备说明

用Tris缓冲盐溶液（TBS）稀释抗-FLAG M2-过氧化物酶溶液：0.05 mM Tris，pH 7.4，含0.15 M NaCl.

其他说明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

法律信息

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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警示用语：

Danger

危险声明

H314,H317,H410

危险分类

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1C - Skin Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Can product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody, be used for immunohistochemistry?

    The product cannot be used on paraffin embedded tissue sections, but it does work on fixed culture cells (as indicated on the Product Information Sheet) and also may work on frozen sections.

  2. What concentration of Product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody, is recommended for my application?

    An antibody titer from 1:100 to 1:1000 is recommended for immunocytochemistry and Western blot.  Minimum antibody titer recommended for ELISA is 1:20,000.  Adjust antibody titer to maximize detection sensitivity and to minimize background.

  3. What are the appropriate storage conditions for Product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody?

    This product ships on wet ice and it is recommended to store the undiluted antibody at 0 to -20°C.  Once diluted, repeated freezing and thawing is not recommended.

  4. I am getting a high background on my Western blots using Product No. A8592, Monoclonal Anti-FLAG® M2-Peroxidase (HRP) antibody.  What can I do to fix this?

    There is a troubleshooting guide provided at the end of the product information sheet.  Some suggestions include the following:  adjusting antibody titer, increase number of washes, increase stringency of washes by addition of 0.05% TWEEN 20 or 0.1% TRITON X-100 to the wash buffers, and/or increase the incubation and wash temperatures. 

  5. How is Product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody, supplied?

    Product No. A8592, ANTI-FLAG M2-peroxidase is supplied as a solution in 10 mM sodium phosphate, pH 7.4, containing 150 mM NaCl and 50% glycerol, plus stabilizer and preservative.  The conjugate protein concentration is approximately 1 mg/ml.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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商品

聚糖标记

蛋白质的结构修饰对于活细胞是必不可少的。发生调节异常时，它们往往是会引发疾病。聚糖是生物系统中许多结构变异的原因，它们在细胞表面的表现通常被称为“糖”。

Glycan Labeling

Glycans play a key role in protein structure and disease; representation on cell surfaces is the glycome.

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