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安全信息

C100

Sigma-Aldrich

过氧化氢酶 来源于牛肝脏

aqueous suspension, 40,000-60,000 units/mg protein (E1%/405)

别名:

H2O2:H2O2 氧化还原酶

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About This Item

CAS号:
9001-05-2
EC 号:
1.11.1.6 (BRENDA, IUBMB)
MDL编号:
MFCD00081483
UNSPSC代码:
12352204
eCl@ss:
32160410
NACRES:
NA.54

表单

aqueous suspension

质量水平

200

比活

40,000-60,000 units/mg protein (E1%/405)

分子量

tetramer ~250 kDa

等电点

5.4

UniProt登记号

P00432

运输

wet ice

储存温度

2-8°C

SMILES字符串

O(CC)C(=O)c1ccc(cc1)O

InChI

1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3

InChI key

NUVBSKCKDOMJSU-UHFFFAOYSA-N

基因信息

cow ... CAT(280743)

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一般描述

研究领域：细胞信号传导

牛肝过氧化氢酶是由4个相同亚基组成的四聚体，每个亚基的分子量为60 kDa。每个亚基都含有与原血红素IX基团结合的铁。过氧化氢酶也能紧密结合NADP，其中NADP和血红素基团的距离在13.7埃之内。

应用

过氧化氢酶作为天然抗氧化剂，可用于研究活性氧物质对基因表达和凋亡的作用。它还被用于保护蛋白质、脂质和核酸免受氧化损伤。工业上，过氧化氢酶已被用于去除添加至牛奶和奶酪中以及纺织品漂白使用的过氧化氢，并用于研究其对大肠杆菌DNA修复突变体生活力的正向效应。

牛肝过氧化氢酶可用于：

  • 制备用于葡萄糖生物燃料电池的H2O2-O2基生物阴极
  • 研究固定在硅酸镁载体上的过氧化氢酶的动力学性质和储存稳定性
  • 水溶液中谷胱甘肽介导的超氧化物生成

生化/生理作用

过氧化氢酶是一种存在于所有需氧生物中的抗氧化酶，可将代谢过程的副产物过氧化氢催化降解为危害较小的水和氧气。它还能与烷基过氧化氢反应，如甲基过氧化物和乙基过氧化物，可以用甲醇、乙醇、丙醇、甲酸盐和硝酸盐作为氢供体取代第二 H2O2 分子。过氧化氢酶使用铁（Fe）或锰（Mn）作为辅因子，分为Fe-CAT或Mn-CAT。

注意

过氧化氢酶溶液不可冷冻。 冷冻溶液将导致酶活性损失50-70％。

单位定义

一单位酶可在pH7.0、25℃条件下每分钟分解1.0 μM的H2O2，通过测量A240的下降速率可知H2O2的浓度从10.3降至9.2 mM。

制备说明

本品为含0.1%（w/v）百里香酚的过氧化氢酶水悬浮液，活性为40,000-60,000单位/mg蛋白质。为除去百里香酚防腐剂，可以通过离心使过氧化氢酶晶体沉淀，弃去上清液，将沉淀物重悬于水中，然后再次沉淀。该酶溶于50 mM磷酸钾缓冲液中，浓度为1 mg/mL，pH 7.0。

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象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

动植物源性产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCP4294
SLCG5609
SLCH7352
SLCC3864
SLBS1139V

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Can Product C100, Catalase from bovine liver, be shipped on wet ice or dry ice?

    Product No. C100 normally ships at ambient temperature. If desired, it can be shipped on wet ice upon special request. C100 cannot be shipped on dry ice. That is, the suspension can be harmed by freezing.

  6. Is Product C100, Catalase from bovine liver, a solution or a suspension?

    Most of the protein (catalase) in Product No. C100 is a solid in suspension, with a small percentage actually in solution.

  7. Is Product C100, Catalase from bovine liver, sterile?

    C100 is not necessarily sterile. But it does contain a preservative (0.1% thymol), which should prevent microbial growth.

  8. What is the catalase concentration of Product C100, Catalase from bovine liver?

    There is no "official" specification, but the lot history shows that it has varied from 15-100 mg/mL. The concentration is provided on the certificate of analysis for each lot. Note that most of the catalase is typically a solid in suspension, with a small percentage actually in solution.

  9. Your website shows that Product No. C100 is catalase. But I have some notes that indicate that C100 is your product number for calmidazolium chloride. Which one is right?

    C-100 was used as a product number for calmidazolium chloride until about 1999 by Research Biochemicals International (RBI), which is now part of Sigma-Aldrich. Calmidazolium chloride is currently listed under Sigma Product No. C3930. So now we use the C100 only for catalase.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Selective lowering of Abeta42 levels (the 42-residue isoform of the amyloid-beta peptide) with small-molecule gamma-secretase modulators (GSMs), such as some non-steroidal anti-inflammatory drugs, is a promising therapeutic approach for Alzheimer's disease. To identify the target of these agents we developed
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Pulmonary infections are a major global cause of morbidity, exacerbated by an increasing threat from antibiotic-resistant pathogens. In this context, therapeutic interventions aimed at protectively modulating host responses, to enhance defence against infection, take on ever greater significance. Pseudomonas aeruginosa
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Scientific reports, 8(1), 10418-10418 (2018-07-12)
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实验方案

Enzymatic Assay of Catalase (EC 1.11.1.6)

This procedure may be used for all Catalase products.

过氧化氢酶的酶学测定（EC 1.11.1.6）

过氧化氢酶催化过氧化氢生成水和氧气。该实验方案使用分光光度法测定过氧化氢酶活性。

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