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C0875

Sigma-Aldrich

钙黄绿素

Powder

别名:

双[N,N−双(羧甲基)氨基甲基]荧光素, 荧光素-双（甲亚胺二乙酸）, 荧光素络合酮

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About This Item

经验公式（希尔记法）:
C30H26N2O13
CAS号:
154071-48-4
分子量:
622.53
Beilstein:
8181068
UNSPSC代码:
12171500
PubChem化学物质编号:
24892279
NACRES:
NA.47

product name

钙黄绿素, Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.

质量水平

200

描述

mixed isomers

形式

powder

技术

titration: suitable

颜色

yellow to brown

溶解性

NaOH: 50 mg/mL

荧光

λex 470 nm; λem 509 nm (pH 7.4)

适用性

suitable for (Suitable for fluorometric determination of Ca Suitable for EDTA titration of Ca in the presence of Mg)

应用

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

SMILES字符串

OC(=O)CN(CC(O)=O)Cc1cc2c(Oc3cc(O)c(CN(CC(O)=O)CC(O)=O)cc3C24OC(=O)c5ccccc45)cc1O

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应用

钙黄绿素已被用于：
  • 全细胞染色
  • 组织形态学和微计算机断层扫描
  • 标记活性骨形成表面

生化/生理作用

钙黄绿素是一种钙依赖型荧光分子。它主要用于研究 体内 条件下的骨代谢，并有助于在 体外 条件下对凹陷区域进行染色。钙黄绿素被用于钙的荧光测定以及钙的EDTA（乙二胺四乙酸）滴定。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What are the excitation and emission wavelengths of Calcein, Product C0875?

    Conn's Biological Stains, 10th Edition states excitation is at 494 nm in aqueous solutions at pH 9; the emission maximum is 517 nm. Depending upon the pH of the solution, slightly different values have been reported in the literature.

  4. Is there a reference for using Calcein, Product C0875, to study bone growth?

    Clement-Lacroix, P., et al. Osteoblasts are a new target for prolactin: analysis of bone formation in prolactin receptor knockout mice. Endocrinology, 140, 96-105 (1999).

  5. What is the extinction coefficient of Calcein, Product C0875?

    For samples in 1 M sodium hydroxide: E1% = 1040 at 500-504 nm; E1% = 466 at 242-245 nm.

  6. What is the solubility of Calcein, Product C0875?

    Calcein is soluble at a concentration of 50 mg/mL in 1M sodium hydroxide, yielding clear orange to brown solution.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Miao D, et al.
Endocrinology, 145, 3554-3554 (2004)
Calcium: Chemistry, Analysis, Function and Effects (2015)
Skeletal abnormalities in Pth-null mice are influenced by dietary calcium.
Miao D, et al.
Endocrinology, 145, 2046-2046 (2004)
South African Topical Ophthalmic Drug Guide (2013)
Kengo Sato et al.
Clinical science (London, England : 1979), 133(16), 1779-1796 (2019-08-11)
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