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产品名称
脑源性神经营养因子 人, BDNF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
生物来源
human
质量水平
重组
expressed in E. coli
方案
≥95% (SDS-PAGE)
表单
lyophilized powder
质量
endotoxin tested
分子量
~27 kDa
包装
pkg of 10 μg
pkg of 5 μg
储存条件
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
技术
cell culture | mammalian: suitable
杂质
≤1.00 EU/μg Endotoxin level
UniProt登记号
储存温度
−20°C
一般描述
BDNF是生长因子中神经营养蛋白家族的成员，该家族还包括NGF、NT-3和NT-4。所有神经营养因子具有六个保守的半胱氨酸残基，并且在氨基酸水平上具有55％的序列同一性。BDNF已被证明可以增强体外几类神经元的存活和分化，包括神经嵴和基板衍生的感觉神经元、黑质中的多巴胺能神经元、基底前脑胆碱能神经元、海马神经元和视网膜神经节细胞.1 BDNF在外周神经节内表达，并且不限于神经元靶区域，因而提高了BDNF对神经元以及非神经元细胞具有旁分泌或甚至自分泌作用的可能性。
人重组BDNF蛋白（27kDa）的活性形式是由两个相同的119个氨基酸亚基形成的二聚体，它们通过强疏水相互作用结合在一起
人重组BDNF蛋白（27kDa）的活性形式是由两个相同的119个氨基酸亚基形成的二聚体，它们通过强疏水相互作用结合在一起
应用
脑源性神经营养因子（BDNF）重组人蛋白已被用作各种培养基中的补充剂。
生化/生理作用
脑源性神经营养因子（BDNF）（13.6kDa或27.2kDa二聚体）是神经营养蛋白家族的成员。 BDNF在人、小鼠和猪中具有相同的氨基酸序列，具有完全的交叉反应性。 BDNF在中枢神经系统内的神经元群体或与其直接相关的细胞的发育和维持中具有重要作用。 在体外，BDNF已报到可增强几类神经元的存活和分化，其中包括神经嵴和基板衍生的感觉神经元、黑质中的多巴胺能神经元、基底前脑胆碱能神经元、海马神经元和视网膜神经节细胞。
外形
从经过无菌0.2 μm过滤的BSA溶液中冻干得到
分析说明
其生物活性由其刺激人神经母细胞瘤细胞SH-SY5Y增殖的能力来确定。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
监管及禁止进口产品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Lisa Oliver et al.
Stem cells and development, 21(15), 2779-2788 (2012-04-24)
Autophagy plays an important role in homeostasis, development, and disease, functioning both as a survival and cell death pathway. However, despite its importance in cell physiology, there is little information about the role of autophagy in stem cells and, in
Differentiation and histological analysis of embryonic stem cell-derived neural transplants in mice.
Y Benninger et al.
Brain pathology (Zurich, Switzerland), 10(3), 330-341 (2000-07-08)
We report here that neural transplantation of in vitro-differentiated embryonic stem (ES) cells provides a versatile strategy for gene transfer into the central nervous system. ES cells were subjected to an optimized in vitro differentiation protocol to obtain embryoid bodies.
W Sun et al.
Neuroscience, 164(4), 1854-1866 (2009-09-26)
Cochlear spiral ganglion neurons (SGN) provide the only pathway for transmitting sound evoked activity from the hair cells to the central auditory system. Neurotrophic factor 3 (NT-3) and brain derived neurotrophic factor (BDNF) released from hair cells and supporting cells
Bo Tian et al.
Nature cell biology, 11(2), 211-218 (2009-01-20)
The phosphatidylinositol-3-kinase-like kinase ATM (ataxia-telangiectasia mutated) has a central role in coordinating DNA damage responses, including cell-cycle checkpoint control, DNA repair and apoptosis. Mutations of ATM cause a spectrum of defects ranging from neurodegeneration to cancer predisposition. However, the mechanism
Lisa Oliver et al.
PloS one, 6(5), e19820-e19820 (2011-05-19)
Adult mesenchymal stem cells (MSCs) can be maintained over extended periods of time before activation and differentiation. Little is known about the programs that sustain the survival of these cells. Undifferentiated adult human MSCs (hMSCs) did not undergo apoptosis in
实验方案
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
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