产品名称
抗-人免疫球蛋白G4-生物素抗体,小鼠单克隆, clone HP-6025, purified from hybridoma cell culture
biological source
mouse
conjugate
biotin conjugate
antibody form
purified immunoglobulin
antibody product type
secondary antibodies
clone
HP-6025, monoclonal
form
buffered aqueous solution
species reactivity
human
technique(s)
direct ELISA: 1:60,000
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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Application
IUIS/WHO2 研究挑选出该单克隆抗体作为最广泛适用的 IgG4 特异性单克隆抗体之一。单克隆抗人 IgG4 可用于通过各种免疫测定法鉴定 IgG4 亚类,包括:ELISA、印迹免疫固定法 (IIF)、免疫荧光测定法 (IFMA)、血凝法 (HA)、血凝抑制法 (HAI)、颗粒计数免疫测定法 (PACIA) 和胞浆 IgG 的检测。
小鼠抗人IgG4生物素单克隆抗体可以1:15,000的工作稀释度用于ELISA。用于ELISA法检测疟原虫感染后产生的血清IgG4。
抗人IgG4-生物素抗体、小鼠单克隆抗体可用于免疫组化和免疫染色。
Biochem/physiol Actions
IgG4是人血清中含量最低的一种免疫球蛋白。IgG4参与独特的fab臂交换(FAE)过程,并限制免疫复合物的形成。 它具有抗炎作用。IgG4是一种治疗性抗体。它可以防止过敏,防止肥大细胞脱颗粒,对抗恶性黑色素瘤。IgG4或与自身免疫性疾病有关,如1型和2型自身免疫性胰腺炎(AIP)、Mikulitz综合征、Kuttner′s肿瘤、Riedel′s甲状腺炎和肾小管间质性肾炎。
IgG抗体亚型是免疫系统中最丰富的血清免疫球蛋白。它由B细胞分泌,存在于血液和细胞外液中,可防止细菌、真菌和病毒引起的感染。母体IgG可通过胎盘转移至胎儿,胎盘对新生儿免疫防御感染至关重要。生物素与人IgG(Fc特异性)单克隆抗体偶联,可结合各种标记物,如抗生物素蛋白或链霉亲和素。
单克隆抗人 IgG4 对 IgG4 亚类具有特异性,在 ELISA 中与 IgG1、IgG2 和 IgG3 不反应。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Other Notes
Physical form
0.01 M 磷酸盐缓冲液 (pH 7.4) 中的溶液,含 1% 牛血清白蛋白和 15 mM 叠氮化钠
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Hayder A Giha et al.
Malaria journal, 8, 306-306 (2009-12-24)
Plasmodium falciparum malaria is a complex disease in which genetic and environmental factors influence susceptibility. IgG isotypes are in part genetically controlled, and GM/KM allotypes are believed to be involved in this control. In this study, 216 individuals from Daraweesh
A Nasr et al.
Scandinavian journal of immunology, 74(4), 390-396 (2011-06-08)
The anti-malarial IgG immune response during the lengthy and dry season in areas of low malaria transmission as in Eastern Sudan is largely unknown. In this study, ELISA was used for the measurement of pre-existing total IgG and IgG subclasses
A Nasr et al.
Scandinavian journal of immunology, 66(1), 87-96 (2007-06-26)
In a prospective clinical study in New Halfa Teaching Hospital, the possible association between FcgammaRIIa-R/H131 polymorphism and anti-malarial antibody responses with clinical outcome of Plasmodium falciparum malaria among Sudanese patients was investigated. A total of 256 individuals were consecutively enrolled
Amre Nasr et al.
BMC immunology, 14, 38-38 (2013-08-15)
C-reactive protein (CRP) is a nonspecific, acute-phase protein that rises in response to infectious and non-infectious inflammatory processes. Infections are the single largest cause of neonatal deaths globally.The primary aim of this study is to examine the association between CRP
Association of immunoglobulin G4 and free light chain with idiopathic pleural effusion
Murata Y, et al.
Clinical and Experimental Immunology, 190(1), 133-142 (2017)
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