生物来源
mouse
抗体形式
purified immunoglobulin (purified IgG1 subclass)
克隆
M2, monoclonal
保质期
4 yr
纯化方式
using Protein A
储存温度
−20°C
一般描述
Monoclonal ANTI-FLAG M2 is a purified immunoglobulin, IgG1, monoclonal antibody, purified from culture supernatant of hybridoma cells, that binds to FLAG® fusion proteins. Unlike ANTI-FLAG M1 antibody, the M2 antibody will recognize the FLAG sequence at the N-terminus, Met-N-terminus, C-terminus, or at an internal site of FLAG fusion proteins. Monoclonal ANTI-FLAG M2 is useful for identification and capture of FLAG fusion proteins by common immunological procedures such as Western blots and immunoprecipitation. It is also useful for affinity purification of FLAG fusion proteins when bound to a solid support.
form: solution pH 7.4, containing 15 mM sodium azide
concentration: 3.0-5.0 mg/mL
form: solution pH 7.4, containing 15 mM sodium azide
concentration: 3.0-5.0 mg/mL
应用
IB, IF, IP, FACS, ELISA
Antibody is recommended for use in several applications such as immunoblotting, immunoprecipitation, immunofluorescence, flow cytometry, and ELISA.
Learn more product details in our FLAG® application portal.
Antibody is recommended for use in several applications such as immunoblotting, immunoprecipitation, immunofluorescence, flow cytometry, and ELISA.
Learn more product details in our FLAG® application portal.
包装
polypropylene screw cap vial
制备说明
Dilute the antibody solution from 0.5-10 ug/mL in specified buffer
法律信息
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Weixiang Bian et al.
STAR protocols, 3(3), 101569-101569 (2022-07-26)
Identification of protein interactors is fundamental to understanding their functions. Here, we describe a modified protocol for tandem affinity purification coupled with mass spectrometry (TAP/MS), which includes two-step purification. We detail the S-, 2×FLAG-, and Streptavidin-Binding Peptide (SBP)- tandem tags
Rui Santalla Méndez et al.
Cellular and molecular life sciences : CMLS, 80(10), 306-306 (2023-09-27)
Intracellular vesicle transport is essential for cellular homeostasis and is partially mediated by SNARE proteins. Endosomal trafficking to the plasma membrane ensures cytokine secretion in dendritic cells (DCs) and the initiation of immune responses. Despite its critical importance, the specific
Lirong Xu et al.
Cell reports, 39(2), 110635-110635 (2022-04-14)
Circadian genes such as Clock, Bmal1, Cryptochrome1/2, and Period1/2/3 constitute the precise circadian system. ClockΔ19 is a commonly used mouse model harboring a circadian clock gene mutation, which lacks the EXON-19-encoded 51 amino acids. Previous reports have shown that ClockΔ19
Junmei Cairns et al.
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Cell division cycle 25 (CDC25) dual specificity phosphatases positively regulate the cell cycle by activating cyclin-dependent kinase/cyclin complexes. Here, we demonstrate that in addition to its role in cell cycle regulation, CDC25B functions as a regulator of protein phosphatase 2A
Yuxia Sun et al.
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