推荐产品
等级
Molecular Biology
质量水平
无菌性
non-sterile
方案
≥98% (HPLC)
表单
powder
技术
nucleic acid detection: suitable
溶解性
DMF: soluble
适用性
suitable for substrate for β-galactosidase
储存温度
−20°C
SMILES字符串
OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O
InChI
1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1
InChI key
LINMATFDVHBYOS-MBJXGIAVSA-N
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一般描述
Bluo-Gal是β-半乳糖苷酶的显色底物，用于确定琼脂平板上生长的细菌中是否存在克隆的DNA插入片段。Bluo-Gal设计用于具有lac +表型的重组细菌菌落的蓝白色选择中替代X-Gal。
应用
Blu-Gal适用于选择具有lac +表型的重组细菌菌落。Bluo-Gal适合作为β-半乳糖苷酶底物用于皮肤组织的电镜分析染色，网织红细胞样品和成年小鼠腔静脉的染色。
适用于鉴定lac+细菌菌落的显色底物。5-溴-4-氯-3-吲哚基 β 的替代物-D-吡喃半乳糖苷 (X-Gal)，可产生较深的蓝色。
原理
当Bluo-Gal被β-半乳糖苷酶水解时，所得产物会形成蓝色沉淀。在Bluo-Gal存在的情况下，Lac +菌落的蓝色比X-Gal更深，从而能够区分Lac +和Lac-菌落。
相关产品
产品编号
说明
价格
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
监管及禁止进口产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Leslie M Stone et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 22(11), 4522-4529 (2002-06-01)
Taste buds are a heterogeneous population of cells exhibiting diverse morphological and biochemical characteristics. Because taste buds arise from multiple progenitors, the different types of taste cells may represent distinct lineages. The present study was undertaken to determine the following:
Ammonium transporter C of Dictyostelium discoideum is required for correct prestalk gene expression and for regulating the choice between slug migration and culmination
Kirsten JH, et al.
Developmental Biology, 287(1), 146-156 (2005)
Delineation of matriptase protein expression by enzymatic gene trapping suggests diverging roles in barrier function, hair formation, and squamous cell carcinogenesis
List K, et al.
The American Journal of Pathology, 168(5), 1513-1525 (2006)
H T Yuan et al.
Journal of the American Society of Nephrology : JASN, 11(6), 1055-1066 (2000-05-23)
Angiopoietin-1 (Ang-1) stimulates endothelial and vascular network differentiation through the Tie-2 receptor tyrosine kinase, while Ang-2 modulates this activation in embryo and tumor growth. The nephrogenic pattern of Ang-2 was documented in a mouse strain that expresses the LacZ reporter
Kyoji Horie et al.
Molecular and cellular biology, 23(24), 9189-9207 (2003-12-03)
The use of mutant mice plays a pivotal role in determining the function of genes, and the recently reported germ line transposition of the Sleeping Beauty (SB) transposon would provide a novel system to facilitate this approach. In this study
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