等级
for molecular biology
质量水平
无菌性
non-sterile
检测方案
≥98% (HPLC)
形式
powder
技术
nucleic acid detection: suitable
溶解性
DMF: soluble
适用性
suitable for substrate for β-galactosidase
储存温度
−20°C
SMILES字符串
OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O
InChI
1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1
InChI key
LINMATFDVHBYOS-MBJXGIAVSA-N
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一般描述
Bluo-Gal是β-半乳糖苷酶的显色底物，用于确定琼脂平板上生长的细菌中是否存在克隆的DNA插入片段。Bluo-Gal设计用于具有lac +表型的重组细菌菌落的蓝白色选择中替代X-Gal。
应用
Blu-Gal适用于选择具有lac +表型的重组细菌菌落。Bluo-Gal适合作为β-半乳糖苷酶底物用于皮肤组织的电镜分析染色，网织红细胞样品和成年小鼠腔静脉的染色。
适用于鉴定lac+细菌菌落的显色底物。5-溴-4-氯-3-吲哚基 β 的替代物-D-吡喃半乳糖苷 (X-Gal)，可产生较深的蓝色。
原理
当Bluo-Gal被β-半乳糖苷酶水解时，所得产物会形成蓝色沉淀。在Bluo-Gal存在的情况下，Lac +菌落的蓝色比X-Gal更深，从而能够区分Lac +和Lac-菌落。
相关产品
产品编号
说明
价格
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
监管及禁止进口产品
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