B2904
卤代腺苷基-beta-D-半乳糖苷
reagent for selection of recombinant bacterial clones
别名:
5-溴-3-吲哚基β-D-吡喃半乳糖苷, 卤代腺苷基-beta-D-半乳糖苷
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关于此项目
经验公式（希尔记法）:
C14H16BrNO6
化学文摘社编号:
分子量:
374.18
UNSPSC Code:
12352200
NACRES:
NA.85
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
1550140
产品名称
卤代腺苷基-beta-D-半乳糖苷, reagent for selection of recombinant bacterial clones
InChI
1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1
SMILES string
OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O
InChI key
LINMATFDVHBYOS-MBJXGIAVSA-N
grade
Molecular Biology
sterility
non-sterile
assay
≥98% (HPLC)
form
powder
technique(s)
nucleic acid detection: suitable
solubility
DMF: soluble
suitability
suitable for substrate for β-galactosidase
storage temp.
−20°C
Quality Level
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Application
Blu-Gal适用于选择具有lac +表型的重组细菌菌落。Bluo-Gal适合作为β-半乳糖苷酶底物用于皮肤组织的电镜分析染色，网织红细胞样品和成年小鼠腔静脉的染色。
适用于鉴定lac+细菌菌落的显色底物。5-溴-4-氯-3-吲哚基 β 的替代物-D-吡喃半乳糖苷 (X-Gal)，可产生较深的蓝色。
Biochem/physiol Actions
当Bluo-Gal被β-半乳糖苷酶水解时，所得产物会形成蓝色沉淀。在Bluo-Gal存在的情况下，Lac +菌落的蓝色比X-Gal更深，从而能够区分Lac +和Lac-菌落。
General description
Bluo-Gal是β-半乳糖苷酶的显色底物，用于确定琼脂平板上生长的细菌中是否存在克隆的DNA插入片段。Bluo-Gal设计用于具有lac +表型的重组细菌菌落的蓝白色选择中替代X-Gal。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
涉药品监管产品
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