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安全信息

AA0100

Sigma-Aldrich

氨检测试剂盒

sufficient for 100 assays

别名:

Ammonia Quantitative Test Kit

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About This Item

UNSPSC代码:
12352202
NACRES:
NA.84

用途

sufficient for 100 assays

质量水平

200

储存温度

2-8°C

应用

在一项评估铬代谢性酸中毒的响应过程中肾氨转运体表达情况的研究中，用户使用了Sigma氨检测试剂盒来测定血清和尿液中的氨浓度。
本试剂盒可用于酶促定量分析、测定食品及其他生物材料中的氨水平。 L-谷氨酸脱氢酶(GIDH)存在的情况下，氨能够与α-酮戊二酸(KGA)和还原型烟酰胺腺嘌呤二核苷酸磷酸盐(NADPH)反应，生成L-谷氨酸和氧化型烟酰胺腺嘌呤二核苷酸磷酸盐(NADP+)。340 nm波长处吸光值下降是NADPH的氧化所致，具体的吸光度与样品中的氨浓度成正比。 L-谷氨酸脱氢酶能够与氨特异性地反应。氨检测试剂盒可用于测定0.2-15 μg/ml的氨浓度。

仅试剂盒组分

产品编号
说明
  • Ammonia Assay Reagent 10 vial(s)
  • L-Glutamate Dehydrogenase for Ammonia Assay 1 mL/vial
  • Ammonia Standard Solution 1 mL/vial

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H319,H334

危险分类

Eye Irrit. 2 - Resp. Sens. 1

储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

监管及禁止进口产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How can I assess the absorbance change of my reagent blank when using Product AA0100, Ammonia Assay Kit?

    Generally, an absorbance change of 0.050 or less indicates an acceptable range for the reagent blank. An absorbance change higher than this indicates possible contamination of the reagent (or glassware) with ammonia.

  4. Is there a control that can be used to assess the accuracy of Product AA0100, Ammonia Assay Kit?

    A convenient control for use with this kit is the Ammonia Standard Solution that is provided in the kit.

  5. Which samples should be deproteinized for use with Product AA0100, Ammonia Assay Kit?

    The basis for this kit was a Diagnostic procedure for the determination of Ammonia in human plasma, whose total protein concentration is approximately 63 to 83 mg/mL. Thus, any solution whose protein concentration is less than approximately 83 mg/mL should not require deproteinization.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Kahina Mellahi et al.
Bioprocess and biosystems engineering, 42(5), 711-725 (2019-01-24)
Mammalian-inducible expression systems are increasingly available and offer an attractive platform for the production of recombinant proteins. In this work, we have conducted process development for a cumate-inducible GS-CHO cell-line-expressing rituximab. To cope with the limitations encountered in batch when
G C Santos et al.
Scientific reports, 7(1), 9510-9510 (2017-08-27)
We combined
Na Gao et al.
Fish & shellfish immunology, 65, 226-234 (2017-04-22)
Ammonia is highly toxic to aquatic organisms, but whether ammonia excretion or ammonia metabolism to less toxic compounds is the major strategy for detoxification in marine fish against chronic ammonia exposure is unclear to date. In this study, we investigated
Junpeng Jiang et al.
BMC plant biology, 19(1), 239-239 (2019-06-07)
Ammonium transporters (AMTs), a family of proteins transporting ammonium salt and its analogues, have been studied in many aspects. Although numerous studies have found that ammonium affects the interaction between plants and pathogens, the role of AMTs remains largely unknown
Tingting Xiang et al.
Nature communications, 11(1), 108-108 (2020-01-09)
In cnidarian-Symbiodiniaceae symbioses, algal endosymbiont population control within the host is needed to sustain a symbiotic relationship. However, the molecular mechanisms that underlie such population control are unclear. Here we show that a cnidarian host uses nitrogen limitation as a
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