推荐产品
生物来源
mouse
质量水平
偶联物
CY3 conjugate
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
M2, monoclonal
表单
buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)
种属反应性
all
浓度
~1 mg/mL
技术
direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
同位素/亚型
IgG1
免疫原序列
DYKDDDDK
运输
dry ice
储存温度
−20°C
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一般描述
单克隆抗标记 M2-Cy3（小鼠IgG）抗体与花菁染料 Cy3 共价偶联。抗体缀合物结合FLAG融合蛋白，并且将在FLAG融合蛋白的N末端、Met-N末端或C末端以识别FLAG序列。
应用
此抗体已成功使用的应用，以及相关的同行评审论文如下。
蛋白免疫分析（Western Blotting）（1篇文章）
蛋白免疫分析（Western Blotting）（1篇文章）
外形
溶于磷酸盐缓冲盐水，含有 1%BSA 和防腐剂
制备说明
用 Tris 缓冲盐水 (TBS) 稀释抗体：0.05 MTris，pH 7.4，含 0.15 M NaCl。
其他说明
免疫细胞化学的建议浓度为1-10 mg / ml。
法律信息
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Cy3 is a trademark of Cytiva
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Oliver Wicht et al.
Journal of virology, 88(9), 4943-4952 (2014-02-21)
Enveloped viruses carry highly specialized glycoproteins that catalyze membrane fusion under strict spatial and temporal control. To prevent premature activation after biosynthesis, viral class I fusion proteins adopt a locked conformation and require proteolytic cleavage to render them fusion-ready. This
Takeshi Yoshizumi et al.
Biomacromolecules, 19(5), 1582-1591 (2018-03-31)
Selective gene delivery into organellar genomes (mitochondrial and plastid genomes) has been limited because of a lack of appropriate platform technology, even though these organelles are essential for metabolite and energy production. Techniques for selective organellar modification are needed to
Robert S Ohgami et al.
Blood, 108(4), 1388-1394 (2006-04-13)
Iron and copper are essential for all organisms, assuming critical roles as cofactors in many enzymes. In eukaryotes, the transmembrane transport of these elements is a highly regulated process facilitated by the single electron reduction of each metal. Previously, we
Yanpu He et al.
Science advances, 6(24), eaba7589-eaba7589 (2020-06-26)
The stimulator of interferon (IFN) genes (STING) pathway constitutes a highly important part of immune responses against various cancers and infections. Consequently, administration of STING agonists such as cyclic GMP-AMP (cGAMP) has been identified as a promising approach to target
Kunio Yokoyama et al.
The Journal of biological chemistry, 286(7), 5375-5382 (2010-12-21)
Rab27a, a Rab family small GTPase, is involved in the exocytosis of secretory granules in melanocytes and cytotoxic T-cells. Rab27a mutations cause type 2 Griscelli syndrome, which is characterized by immunodeficiency, including uncontrolled macrophage activation known as hemophagocytic syndrome. However
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