推荐产品
生物来源
mouse
质量水平
偶联物
CY3 conjugate
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
M2, monoclonal
表单
buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)
种属反应性
all
浓度
~1 mg/mL
技术
direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
同位素/亚型
IgG1
免疫原序列
DYKDDDDK
运输
dry ice
储存温度
−20°C
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一般描述
单克隆抗标记 M2-Cy3（小鼠IgG）抗体与花菁染料 Cy3 共价偶联。抗体缀合物结合FLAG融合蛋白，并且将在FLAG融合蛋白的N末端、Met-N末端或C末端以识别FLAG序列。
应用
此抗体已成功使用的应用，以及相关的同行评审论文如下。
蛋白免疫分析（Western Blotting）（1篇文章）
蛋白免疫分析（Western Blotting）（1篇文章）
外形
溶于磷酸盐缓冲盐水，含有 1%BSA 和防腐剂
制备说明
用 Tris 缓冲盐水 (TBS) 稀释抗体：0.05 MTris，pH 7.4，含 0.15 M NaCl。
其他说明
免疫细胞化学的建议浓度为1-10 mg / ml。
法律信息
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Cy3 is a trademark of Cytiva
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Saifeng Wang et al.
Genes & development, 33(15-16), 1048-1068 (2019-06-22)
Fetal hematopoietic stem and progenitor cells (HSPCs) hold promise to cure a wide array of hematological diseases, and we previously found a role for the RNA-binding protein (RBP) Lin28b in respecifying adult HSPCs to resemble their fetal counterparts. Here we
Oliver Wicht et al.
Journal of virology, 88(9), 4943-4952 (2014-02-21)
Enveloped viruses carry highly specialized glycoproteins that catalyze membrane fusion under strict spatial and temporal control. To prevent premature activation after biosynthesis, viral class I fusion proteins adopt a locked conformation and require proteolytic cleavage to render them fusion-ready. This
Robert S Ohgami et al.
Blood, 108(4), 1388-1394 (2006-04-13)
Iron and copper are essential for all organisms, assuming critical roles as cofactors in many enzymes. In eukaryotes, the transmembrane transport of these elements is a highly regulated process facilitated by the single electron reduction of each metal. Previously, we
Takeshi Yoshizumi et al.
Biomacromolecules, 19(5), 1582-1591 (2018-03-31)
Selective gene delivery into organellar genomes (mitochondrial and plastid genomes) has been limited because of a lack of appropriate platform technology, even though these organelles are essential for metabolite and energy production. Techniques for selective organellar modification are needed to
Kunio Yokoyama et al.
The Journal of biological chemistry, 286(7), 5375-5382 (2010-12-21)
Rab27a, a Rab family small GTPase, is involved in the exocytosis of secretory granules in melanocytes and cytotoxic T-cells. Rab27a mutations cause type 2 Griscelli syndrome, which is characterized by immunodeficiency, including uncontrolled macrophage activation known as hemophagocytic syndrome. However
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