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Merck
CN

A9594

单克隆抗-FLAG® M2-Cy3 小鼠抗

clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

别名:

单克隆抗-FLAG® M2 小鼠抗, 抗 ddddk, 抗 dykddddk

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关于此项目

NACRES:
NA.32
UNSPSC Code:
41106514
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产品名称

单克隆抗-FLAG® M2-Cy3 小鼠抗, clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

biological source

mouse

conjugate

CY3 conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

species reactivity

all

concentration

~1 mg/mL

technique(s)

direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

Quality Level

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Application

实现简单的一步式免疫细胞化学检测。 特别适用于小鼠宿主表达FLAG融合蛋白的检测,这种检测使用抗小鼠二抗可能会发生交叉反应。

请访问我们的FLAG®应用页面,了解更多产品详情。
此抗体已成功使用的应用,以及相关的同行评审论文如下。
蛋白免疫分析(Western Blotting)(1篇文章)

General description

单克隆抗标记 M2-Cy3(小鼠IgG)抗体与花菁染料 Cy3 共价偶联。抗体缀合物结合FLAG融合蛋白,并且将在FLAG融合蛋白的N末端、Met-N末端或C末端以识别FLAG序列。

Other Notes

免疫细胞化学的建议浓度为1-10 mg / ml。

Physical form

溶于磷酸盐缓冲盐水,含有 1%BSA 和防腐剂

Preparation Note

用 Tris 缓冲盐水 (TBS) 稀释抗体:0.05 MTris,pH 7.4,含 0.15 M NaCl。

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Cy3 is a trademark of Cytiva
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Oliver Wicht et al.
Journal of virology, 88(9), 4943-4952 (2014-02-21)
Enveloped viruses carry highly specialized glycoproteins that catalyze membrane fusion under strict spatial and temporal control. To prevent premature activation after biosynthesis, viral class I fusion proteins adopt a locked conformation and require proteolytic cleavage to render them fusion-ready. This
Takeshi Yoshizumi et al.
Biomacromolecules, 19(5), 1582-1591 (2018-03-31)
Selective gene delivery into organellar genomes (mitochondrial and plastid genomes) has been limited because of a lack of appropriate platform technology, even though these organelles are essential for metabolite and energy production. Techniques for selective organellar modification are needed to
Robert S Ohgami et al.
Blood, 108(4), 1388-1394 (2006-04-13)
Iron and copper are essential for all organisms, assuming critical roles as cofactors in many enzymes. In eukaryotes, the transmembrane transport of these elements is a highly regulated process facilitated by the single electron reduction of each metal. Previously, we
Yanpu He et al.
Science advances, 6(24), eaba7589-eaba7589 (2020-06-26)
The stimulator of interferon (IFN) genes (STING) pathway constitutes a highly important part of immune responses against various cancers and infections. Consequently, administration of STING agonists such as cyclic GMP-AMP (cGAMP) has been identified as a promising approach to target
Ruizhi Feng et al.
Journal of medical genetics, 53(10), 662-671 (2016-06-09)
TUBB8 is a primate-specific β-tubulin isotype whose expression is confined to oocytes and the early embryo. We previously found that mutations in TUBB8 caused oocyte maturation arrest. The objective was to describe newly discovered mutations in TUBB8 and to characterise

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