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安全信息

A9594

Sigma-Aldrich

单克隆抗-FLAG® M2-Cy3™ 小鼠抗

clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

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别名:
单克隆抗-FLAG® M2 小鼠抗, 抗 ddddk, 抗 dykddddk
UNSPSC代码:
41106514
NACRES:
NA.32

生物来源

mouse

质量水平

200

偶联物

CY3 conjugate

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

M2, monoclonal

形式

buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

种属反应性

all

浓度

~1 mg/mL

技术

direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone

同位素/亚型

IgG1

免疫原序列

DYKDDDDK

运输

dry ice

储存温度

−20°C

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相关类别

一般描述

单克隆抗标记 M2-Cy3（小鼠IgG）抗体与花菁染料 Cy3 共价偶联。抗体缀合物结合FLAG融合蛋白，并且将在FLAG融合蛋白的N末端、Met-N末端或C末端以识别FLAG序列。

应用

实现简单的一步式免疫细胞化学检测。 特别适用于小鼠宿主表达FLAG融合蛋白的检测，这种检测使用抗小鼠二抗可能会发生交叉反应。

请访问我们的FLAG®应用页面，了解更多产品详情。
此抗体已成功使用的应用，以及相关的同行评审论文如下。
蛋白免疫分析（Western Blotting）（1篇文章）

外形

溶于磷酸盐缓冲盐水，含有 1%BSA 和防腐剂

制备说明

用 Tris 缓冲盐水 (TBS) 稀释抗体：0.05 MTris，pH 7.4，含 0.15 M NaCl。

其他说明

免疫细胞化学的建议浓度为1-10 mg / ml。

法律信息

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Cy3 is a trademark of Cytiva
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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