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安全信息

A9528

Sigma-Aldrich

两性霉素B，可溶

powder, suitable for cell culture, BioXtra

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别名:
两性霉素B 来源于链霉菌 属, Fungizone
经验公式（希尔记法）:
C47H73NO17
CAS号:
1397-89-3
分子量:
924.08
Beilstein:
78342
MDL编号:
MFCD00877763
UNSPSC代码:
12352207
eCl@ss:
34010400
PubChem化学物质编号:
24891468
NACRES:
NA.76

质量水平

300

无菌性

γ-irradiated

产品线

BioXtra

形式

powder

技术

cell culture | mammalian: suitable

抗生素抗菌谱

fungi
yeast

作用机制

cell membrane | interferes

储存温度

2-8°C

SMILES字符串

C[C@H]1O[C@@H](O[C@@H]2C[C@@H]3O[C@](O)(C[C@@H](O)C[C@@H](O)[C@H](O)CC[C@@H](O)C[C@@H](O)CC(=O)O[C@@H](C)[C@H](C)[C@H](O)[C@@H](C)\C=C\C=C\C=C\C=C\C=C\C=C\C=C\2)C[C@H](O)[C@H]3C(O)=O)[C@@H](O)[C@@H](N)[C@@H]1O

InChI

1S/C47H73NO17/c1-27-17-15-13-11-9-7-5-6-8-10-12-14-16-18-34(64-46-44(58)41(48)43(57)30(4)63-46)24-38-40(45(59)60)37(54)26-47(61,65-38)25-33(51)22-36(53)35(52)20-19-31(49)21-32(50)23-39(55)62-29(3)28(2)42(27)56/h5-18,27-38,40-44,46,49-54,56-58,61H,19-26,48H2,1-4H3,(H,59,60)/b6-5+,9-7+,10-8+,13-11+,14-12+,17-15+,18-16+/t27-,28-,29-,30+,31+,32+,33-,34-,35+,36+,37-,38-,40+,41-,42+,43+,44-,46-,47+/m0/s1

InChI key

APKFDSVGJQXUKY-INPOYWNPSA-N

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相关类别

一般描述

化学结构：多烯

应用

两性霉素B是来自链霉菌属的一种多烯抗真菌抗生素。它对真菌和细菌细胞膜的固醇，尤其是麦角固醇具有很高的亲和力。 维持抗真菌细胞培养的正常用量为2.5 mg/L，同时在培养基中使用青霉素和链霉素。

生化/生理作用

两性霉素B可与甾醇结合，在膜中形成孔，并导致小分子的泄漏。该产品对真菌和酵母最有效。

包装

可溶性两性霉素B包装在琥珀色玻璃瓶中，内容物置于插入的熔锥内。

组分

本品为两性霉素 B 的胶体混悬液，以去氧胆酸盐为增溶剂。 处方为约 45% 两性霉素 B 和 35% 脱氧胆酸钠的磷酸钠和氯化钠溶液。

注意

两性霉素 B 在 37°C 培养 3 天仍保持活性。在-20°C 条件下，通过防风避光，本品可保持活性 5 年。

制备说明

两性霉素 B 可溶于水 (19.6-20.4 mg/mL)，在自然 pH 下，无需加热，即可得到淡黄色澄清至轻微浑浊的溶液。
水溶液由于溶解性差，不能过滤除菌。 如果在无菌水中复溶，则无需过滤除菌。

也可溶于 DMSO (30-40 mg/mL) 和二甲基甲酰胺 (2-4 mg/mL)。

其他说明

保存于密闭容器内，置于干燥通风处。本品对光和湿度敏感。置于干燥处保存。
根据 UV 纯度计算包装产品的实际固体重量。

仅试剂盒组分

产品编号
说明
  • Amphotericin B ~ 45 %
  • Sodium deoxycholate ~ 35 %
  • Sodium phosphate balance

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H372

危险分类

STOT RE 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

法规信息

农药列管产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the shelf life of Product A9528, Amphotericin B solubilized?

    Product No. A9528 has a recommended retest date of 5 years from the initial QC release date.  This date can be found on the lot-specific Certificate of Analysis.

  6. What is the amount of Amphotericin B in Product No. A9528?

    The package size is the amount of Amphotericin B present in the vial.  If weighing the product, utilize the potency value on the lot specific Certificate of Analysis as a correction factor.

  7. What concentration should Product A9528, Amphotericin B solubilized, be used at?

    We recommend to use Product No. A9528 at 5.6 mg solid per Liter.  Cell culture tested amphotericin B solubilized preparation is approximately 45% amphotericin B, 35% sodium deoxycholate, with the balance being sodium phosphate. The amphotericin B is at least 80% amphotericin B and 3-5% amphotericin A by HPLC.  The exact amount of amphotericin B in the product can be found on the lot specific Certificate of Analysis under Potency.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Derayvia Grimes et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(39), 24403-24414 (2020-09-16)
Calcium signals are initiated in immune cells by the process of store-operated calcium entry (SOCE), where receptor activation triggers transient calcium release from the endoplasmic reticulum, followed by opening of plasma-membrane calcium-release activated calcium (CRAC) channels. ORAI1, ORAI2, and ORAI3
Devin Tran et al.
Cornea, 38(7), 901-904 (2019-04-19)
Fungal infections in lamellar keratoplasty are a growing concern. Optisol-GS does not contain an antifungal agent and supplementation with 0.255 μg/mL Amphotericin B (AmpB) has been considered. This study tested the ability of 0.255 μg/mL AmpB in Optisol-GS to eliminate
Etienne Loire et al.
Heliyon, 6(10), e05146-e05146 (2020-10-22)
Contagious caprine pleuropneumonia is an infectious and contagious disease affecting goats and wildlife ruminants, mostly in Africa and Asia. It is caused by a mycoplasma, Mycoplasma capricolum susbp. capripneumoniae, which is very fastidious. This may be the reason why there
Yuki Yamamoto et al.
Reproduction, fertility, and development, 28(6), 673-681 (2014-11-06)
Endothelin (EDN) is a possible regulating factor of oviductal motility, which is important for the transport of gametes and embryo. To clarify the factors that control the secretion of EDN in the bovine oviduct, the expression of EDNs, EDN-converting enzymes
Nadeeka S Udawatte et al.
Frontiers in pharmacology, 11, 511-511 (2020-05-12)
Candida albicans is the major fungal species associated with superficial mucosal infections such as oral candidiasis as well as systemic mycoses with high morbidity and mortality. On top of the rising drug resistance, currently available antifungal agents have significant adverse
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