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Merck
CN

A9469

Sigma-Aldrich

单克隆抗-FLAG®碱性磷酸酶 小鼠抗

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

别名:

单克隆抗-FLAG® M2 小鼠抗, 抗 ddddk, 抗 dykddddk

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About This Item

UNSPSC代码:
41106514
NACRES:
NA.32

生物来源

mouse

质量水平

偶联物

alkaline phosphatase conjugate

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

M2, monoclonal

形式

buffered aqueous glycerol solution

种属反应性

all

浓度

~1 mg/mL

技术

indirect ELISA: 1:20,000

同位素/亚型

IgG1

免疫原序列

DYKDDDDK

运输

wet ice

储存温度

−20°C

基因信息

human ... ALPL(249)

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一般描述

单克隆ANTI-FLAG® M2碱性磷酸酶是一种纯化的 IgG 1 小鼠抗体,与小牛肠碱性磷酸酶(AP)共价结合。该抗体偶联物可与FLAG®融合蛋白结合,并在融合蛋白的任何位置(N末端、Met-N末端、C末端或内部FLAG®肽)识别FLAG®表位。

应用

小鼠中产生的单克隆 ANTI-FLAG® M2-碱性磷酸酶抗体已用于:
  • 甜橙叶柄样品的直接组织印迹免疫测定
  • δ阿片受体内化的筛选
  • 采用 ELISA 筛查无细胞的蛋白表达

成功使用该抗体的应用以及相关的同行评审论文如下所示。
蛋白质印迹(1篇论文)

外形

在含有50%甘油以及稳定剂和防腐剂的磷酸盐缓冲盐溶液中

法律信息

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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Beth A Rasala et al.
Plant biotechnology journal, 9(6), 674-683 (2011-05-04)
Microalgae have the potential to be a valuable biotechnological platform for the production of recombinant proteins. However, because of the complex regulatory network that tightly controls chloroplast gene expression, heterologous protein accumulation in a wild-type, photosynthetic-competent algal chloroplast remains low.
Seongjoon Kang et al.
Biology, 7(2) (2018-05-09)
Chlamydomonas reinhardtii (Chlamydomonas) strains that are toxic to mosquito larvae because they express chloroplast transgenes that are based on the mosquitocidal proteins of Bacillus thuringiensis subsp. israelensis (Bti) could be very useful in mosquito control. Chlamydomonas has several advantages for
Integration of cell-free protein coexpression with an enzyme-linked immunosorbent assay enables rapid analysis of protein-protein interactions directly from DNA
Layton CJ and Helling HW
Protein Science, 20(8), 1432-1438 (2011)
Jiandong Chen et al.
Genes & development, 31(13), 1382-1395 (2017-08-11)
Mismatch repair (MMR) is a conserved mechanism exploited by cells to correct DNA replication errors both in growing cells and under nongrowing conditions. Hfq (host factor for RNA bacteriophage Qβ replication), a bacterial Lsm family RNA-binding protein, chaperones RNA-RNA interactions
Beth A Rasala et al.
PloS one, 7(8), e43349-e43349 (2012-09-01)
Microalgae have recently received attention as a potential low-cost host for the production of recombinant proteins and novel metabolites. However, a major obstacle to the development of algae as an industrial platform has been the poor expression of heterologous genes

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