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安全信息

A9046

Sigma-Aldrich

抗-鸡 IgY（IgG）（全分子）−过氧化物酶 兔抗

affinity isolated antibody, buffered aqueous solution

别名:

Anti Chicken Igy

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About This Item

MDL编号:
MFCD00162225
UNSPSC代码:
12352203
NACRES:
NA.46

生物来源

rabbit

质量水平

200

偶联物

peroxidase conjugate

抗体形式

affinity isolated antibody

抗体产品类型

secondary antibodies

克隆

polyclonal

形式

buffered aqueous solution

种属反应性

chicken

技术

direct ELISA: 1:30,000
dot blot: 1:160,000 (indirect chemiluminescence)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

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相关类别

一般描述

鸡IgY是鸡血清中的主要抗体。鸡对哺乳动物抗原蛋白产生相对增强的抗体反应。这些免疫球蛋白不与哺乳动物和细菌中的Fc受体结合，也不与类风湿因子相互作用。因此，此抗鸡抗体是各种研究应用中重要的分析工具。
兔抗鸡 IgY (IgG)（全分子）-过氧化物酶抗体与鸡 IgG 结合。

免疫原

纯化鸡 IgG

应用

兔抗鸡IgY(IgG)（全分子）-过氧化物酶抗体可用于免疫沉淀和蛋白质印迹（1:2,000）测定。该抗体还可用于直接ELISA（1:30,000）、斑点印迹（1:12,000-1:15,000）和 IHC（1:1,000）应用。
兔抗鸡IgY（IgG）（全分子）-过氧化物酶抗体用于间接酶联免疫吸附测定（ELISA）、直接ELISA、斑点印迹和免疫组化。
用 HRP 标记的兔抗鸡 IgY 作为二抗，对通过氧化铁磁化从兔肾小球分离的蛋白进行免疫印迹分析。

生化/生理作用

从母鸡体内获得的蛋黄IgY免疫球蛋白已进行广泛研究。可用于免疫检测，确实与类风湿因子相互作用。

外形

溶解于 0.01M 磷酸盐缓冲液（pH7.4, 含0.05% MIT）。

制备说明

用两步法戊二醛法制备（Avrameas, S. 等人，Scand. J. Immunol., 8, Suppl. 7, 7 (1978)）。

免责声明

除非我们的产品目录或产品随附的其他公司文件中另有说明，否则我们的产品仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、体外或体内治疗用途或任何类型的人类或动物消费或应用。

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象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H317

危险分类

Skin Sens. 1

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Will Product A9046, Anti-Chicken IgY, also recognize chicken IgG?

    This antibody (and possibly other anti-chicken antibodies) recognizes both chicken IgG from serum and chicken IgY from egg yolk, because these immunoglobulins are structurally very similar.

  6. How is chicken IgY different from chicken IgG?

    Chicken IgG refers to antibodies from the chicken serum and chicken IgY antibodies are from the yolk.

  7. Product A9046, Anti-Chicken IgY, is peroxidase conjugated.  Is this similar to horseradish peroxidase?

    The peroxidase used to make this product is indeed peroxidase from horseradish (HRP).

  8. Do we have additional anti-chicken IgY conjugates?

    We provide anti-chicken IgY antibodies that are conjugated to alkaline phosphatase (Product No. A9171) and FITC (Product No. F8888).  We also provide the unconjugated anti-chicken IgY antibody (Product No. C2288).

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Yong Li et al.
American journal of physiology. Renal physiology, 282(6), F1129-F1139 (2002-05-09)
The transmembrane sialoglycoprotein podocalyxin is thought to be essential in the fine interdigitating foot process structure of the podocyte. The intracellular COOH-terminal amino acids Asp-Thr-His-Leu (DTHL) of podocalyxin comprise a putative ligand for a type I PSD95-Dlg-zona occludens-1 (PDZ) domain.
Yasmina Noubia Abdiche et al.
mAbs, 8(2), 264-277 (2015-12-15)
The ability of monoclonal antibodies (mAbs) to target specific antigens with high precision has led to an increasing demand to generate them for therapeutic use in many disease areas. Historically, the discovery of therapeutic mAbs has relied upon the immunization
Antal Nyeste et al.
The Journal of biological chemistry, 291(9), 4473-4486 (2016-01-02)
The prion protein (PrP) seems to exert both neuroprotective and neurotoxic activities. The toxic activities are associated with the C-terminal globular parts in the absence of the flexible N terminus, specifically the hydrophobic domain (HD) or the central region (CR).
Production and purification of IgY antibodies from chicken egg yolk.
Amro W A, et al.
Journal of Genetic Engineering and Biotechnology, 16(1), 99-103 (2018)
Germline polymorphisms and survival of lung adenocarcinoma patients: A genome-wide study in two European patient series
Galvan A, et al.
International Journal of Cancer. Journal International Du Cancer, 136(5), E262-E271 (2015)
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