A8919
抗山羊 IgG（全分子）-过氧化物酶 兔抗
IgG fraction of antiserum, buffered aqueous solution
别名:
Anti Goat Hrp Sigma
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关于此项目
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d), IHC (p), WB CL
Citations:
66
biological source
rabbit
conjugate
peroxidase conjugate
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:30,000, dot blot: 1:2,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, western blot (chemiluminescent): 1:160,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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General description
免疫球蛋白是由两条重链和两条轻链通过二硫键连接而成的糖蛋白。免疫球蛋白g（IgG）是一种主要的免疫球蛋白。山羊IgG 有两个子类：IgG1 和IgG2。然后，使用0.2％戊二醛进行蛋白质交联，使兔抗山羊IgG抗体与过氧化物酶偶联。偶联物为溶液形式，溶于0.01M磷酸盐缓冲盐水，pH 7.4，含有15 mM MIT作为防腐剂。
Immunogen
纯化山羊IgG
Application
兔抗山羊IgG（全分子）-过氧化物酶抗体可用于免疫印迹和免疫组织化学。
Biochem/physiol Actions
免疫球蛋白主要用于帮助免疫防御。免疫球蛋白G（IgG）参与II型和III型超敏反应。
Physical form
溶解在0.01M pH7.4磷酸缓冲盐的溶液含有0.05%MIT。
Preparation Note
通过Avrameas, S.等人在Scand. J. Immunol., 8, Suppl. 7, 7 (1978)中描述的两步戊二醛法制备。
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