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质量水平
形式
emulsion (aqueous)
浓度
10% (active silicon)
技术
cell culture | hybridoma: suitable
溶解性
H2O: soluble (effective hot or cold.)
密度
1.0 g/mL at 25 °C
应用
microbiology
储存温度
room temp
适用性
(Mammalian (suspension))
bacteria (fermentation)
一般描述
消泡剂 SE-15 是一种硅树脂产品，用于防止或减少微生物发酵系统和哺乳动物悬浮培养物中的泡沫。泡沫控制 使细胞损伤最小化，导致蛋白/抗体生成增加。
应用
消泡剂 SE-15 可用于
- 制备分支滚环扩增(RAM) 用扩增缓冲溶液。
- 研究其对细胞活力、病毒感染性和PCR（聚合酶链反应）的影响。
- 作为磷酸盐缓冲溶液（PBS）的成分之一，用于通过2,2′-叠氮-双（3-乙基苯并噻唑啉-6磺酸）（ABTS）试验检测总抗氧化状态（TAOS）
- 作为自动诱导表达培养液的成分。
- 作为环磷酸腺苷（cAMP）检测缓冲液的成分。
特点和优势
- 控制微生物培养瓶中的发泡或发酵。
- 对培养基无毒
- 可根据需要泵入发酵罐。
组分
消泡剂 SE-15 是一种含有 10% 活性硅和非离子型乳化剂的水性乳液。
制备说明
消泡剂 SE-15 可用 3-10 份冷水预稀释，以辅助分散。预稀释
混悬液应立即使用。
混悬液应立即使用。
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - STOT RE 2 Inhalation
靶器官
Lungs
WGK
WGK 1
Detection of nucleic acid targets using ramified rolling circle DNA amplification: a single nucleotide polymorphism assay model
Smith J H and Beals T P
PLoS ONE, 8(5), e65053-e65053 (2013)
James H Smith et al.
PloS one, 8(5), e65053-e65053 (2013-06-01)
Isothermal amplification methods provide alternatives to PCR that may be preferable for some nucleic acid target detection tasks. Among current isothermal target detection methods, ramified rolling circle amplification (RAM) of single-stranded DNA circles that are formed by ligation of linear
Elisa Binda et al.
BMC biotechnology, 13, 24-24 (2013-03-19)
VanYn, encoded by the dbv7 gene (also known as vanYn) of the biosynthetic cluster devoted to A40926 production, is a novel protein involved in the mechanism of self-resistance in Nonomuraea sp. ATCC 39727. This filamentous actinomycete is an uncommon microorganism
Anna Katharina Schuh et al.
Redox report : communications in free radical research, 23(1), 83-93 (2017-12-20)
Redox regulation plays a crucial role in balancing the cardiovascular system. In this prospective study we aimed to identify currently unknown correlations valuable to cardiovascular research and patient management. Blood samples from 500 patients were collected directly before cardiosurgical interventions
Dario A Vitturi et al.
American journal of physiology. Heart and circulatory physiology, 296(5), H1398-H1407 (2009-03-17)
Allosteric regulation of nitrite reduction by deoxyhemoglobin has been proposed to mediate nitric oxide (NO) formation during hypoxia. Nitrite is predominantly an anion at physiological pH, raising questions about the mechanism by which it enters the red blood cell (RBC)
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