推荐产品
生物来源
goat
质量水平
偶联物
alkaline phosphatase conjugate
抗体形式
affinity isolated antibody
抗体产品类型
secondary antibodies
克隆
polyclonal
表单
buffered aqueous glycerol solution
技术
direct ELISA: 1:30,000
immunohistochemistry (frozen sections): 1:50
western blot: 1:30,000
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
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一般描述
IgG, a monomer, is the predominant antibody circulating in blood which assist in phagocytic destruction of foreign microorganism. Anti-rat IgG (whole molecule)-alkaline phosphatase antibody (diluted 1: 30 000) can be used in rat MPO (myeloperoxidase) ELISA. It may also use as secondary antibody in immunodetection method. Anti-rat IgG (whole molecule)-alkaline phosphatase antibody reacts specifically with all rat immunoglobulins.
免疫原
Purified rat IgG.
应用
Anti-rat IgG (whole molecule)–alkaline phosphatase antibody can be used in ELISA and western blot assays.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Western blot was performed to analyze purified proteins over expressed in E.coli using alkaline-phosphatase-conjugated goat anti-rat IgG at a 1:1000 dilution. Western blot was developed using 5-bromo-4-chloro-3indolyl phosphate/nitro blue tetrazolium tablets (Sigma).
外形
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 10 mM glycine, 50% glycerol and 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
含少量动物源组分生物产品
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Jianmin Zuo et al.
Journal of virology, 82(5), 2385-2393 (2007-12-21)
The DNase/alkaline exonuclease (AE) genes are well conserved in all herpesvirus families, but recent studies have shown that the AE proteins of gammaherpesviruses such as Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) exhibit an additional function which shuts down
M Poueymiro et al.
mBio, 5(6) (2014-12-30)
The plant pathogen Ralstonia solanacearum possesses two genes encoding a trehalose-6-phosphate synthase (TPS), an enzyme of the trehalose biosynthetic pathway. One of these genes, named ripTPS, was found to encode a protein with an additional N-terminal domain which directs its
Claudia Sala et al.
Molecular microbiology, 71(5), 1102-1116 (2009-01-22)
Comparative genomics with Staphylococcus aureus suggested the existence of a regulatory system governing beta-lactamase (BlaC) production in Mycobacterium tuberculosis. The crystal structure of Rv1846c, a winged helix regulator of previously unknown function, was solved thus revealing strong similarity to the
Y C Patry et al.
Clinical and experimental immunology, 132(3), 505-508 (2003-06-05)
We tested whether rat and human MPO have similar antigenic determinants using 36 human MPO-ANCA positive sera, one mouse anti-rat MPO and four mouse anti-human MPO monoclonal reagents. Purified rat and human MPO were used in ELISA, with or without
J Chang et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 278(1), R209-R214 (2000-01-25)
Heat shock proteins (HSPs) may play a cardioprotective role during hypoxia or ischemia. We hypothesized that cardiac tissue from hypoxia-tolerant animals might have high levels of specific HSPs. We measured myocardial HSP60 and HSP72/73 in painted and softshell turtles during
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