质量水平
形式
emulsion (aqueous)
浓度
100% (organic)
密度
1.01 g/mL at 25 °C
应用
microbiology
储存温度
room temp
适用性
(Mammalian (suspension))
bacteria (fermentation)
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一般描述
消泡剂204是一种有机消泡剂，可用于防止或最小化微生物发酵系统和哺乳动物悬浮培养物中的起泡现象。 控制发泡可以最大限度地减少对细胞的损害，从而增加蛋白质/抗体的产生。
应用
适用于防止或充分减少微生物发酵系统和哺乳动物悬浮培养物中的泡沫。
消泡剂204已用于：
消泡剂204已用于：
- PUL（PLAP、Ufd3p和Lub1p）结构域的表达和纯化
- 分批补料发酵
- 防止Luria Bertani细菌生长培养基中因曝气形成气泡
- 采用矿物培养基分批发酵
组分
消泡剂204是一种100％活性成分，是一种在多元醇分散体中无硅有机消泡剂的混合物。
其他说明
包含 100% 活性成分，是多种无硅酮有机消泡剂的混合物，在多元醇中分散。可反复灭菌。
制备说明
对于在微生物培养基中的使用，Sigma建议起始浓度为0.005％至0.01％。
各种应用所需的最佳消泡剂用量需经过确定。消泡剂204在低于15℃的温度下可溶于甲醇、乙醇、甲苯、二甲苯、全氯乙烯和冷水中。它不溶于温水和乙二醇。
各种应用所需的最佳消泡剂用量需经过确定。消泡剂204在低于15℃的温度下可溶于甲醇、乙醇、甲苯、二甲苯、全氯乙烯和冷水中。它不溶于温水和乙二醇。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves
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Beth Junker
Biotechnology progress, 23(4), 767-784 (2007-06-15)
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