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Merck
CN

A8311

消泡剂 204

aqueous emulsion for bacterial and mammalian systems

别名:

organic antifoam

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关于此项目

UNSPSC Code:
12161901
NACRES:
NA.85
MDL number:
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产品名称

消泡剂 204, aqueous emulsion for bacterial and mammalian systems

form

emulsion (aqueous)

concentration

100% (organic)

density

1.01 g/mL at 25 °C

application(s)

microbiology

storage temp.

room temp

suitability

(Mammalian (suspension))
bacteria (fermentation)

Quality Level

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Application

适用于防止或充分减少微生物发酵系统和哺乳动物悬浮培养物中的泡沫。
消泡剂204已用于:
  • PUL(PLAP、Ufd3p和Lub1p)结构域的表达和纯化
  • 分批补料发酵
  • 防止Luria Bertani细菌生长培养基中因曝气形成气泡
  • 采用矿物培养基分批发酵

General description

消泡剂204是一种有机消泡剂,可用于防止或最小化微生物发酵系统和哺乳动物悬浮培养物中的起泡现象。 控制发泡可以最大限度地减少对细胞的损害,从而增加蛋白质/抗体的产生。

Other Notes

包含 100% 活性成分,是多种无硅酮有机消泡剂的混合物,在多元醇中分散。可反复灭菌。
消泡剂204是一种100%活性成分,是一种在多元醇分散体中无硅有机消泡剂的混合物。

Preparation Note

对于在微生物培养基中的使用,Sigma建议起始浓度为0.005%至0.01%。
各种应用所需的最佳消泡剂用量需经过确定。消泡剂204在低于15℃的温度下可溶于甲醇、乙醇、甲苯、二甲苯、全氯乙烯和冷水中。它不溶于温水和乙二醇。

存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


历史批次信息供参考:

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Medium redesign for stable cultivation and high production of mevalonate by recombinant Methtylobacterium extorquens AM1 with mevalonate synthetic pathway
Cui L Y, et al.
Biochemical Engineering Journal, 119, 67-73 (2017)
Beth Junker
Biotechnology progress, 23(4), 767-784 (2007-06-15)
Key aspects of foaming and its mitigation in fermentation systems are presented. Foam properties and behavior, conditions that affect foaming, and consequences of foaming are discussed, followed by methods to detect and prevent foam, both without and with the use
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The development of robust and efficient cell factories requires understanding of the metabolic changes triggered by the production of the targeted compound. Here we aimed to study how production of p-coumaric acid, a precursor of multiple secondary aromatic metabolites, influences
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