生物来源
goat
质量水平
偶联物
peroxidase conjugate
抗体形式
IgG fraction of antiserum
抗体产品类型
secondary antibodies
克隆
polyclonal
表单
buffered aqueous solution
技术
direct ELISA: 1:10,000
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
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一般描述
IgG是一种单体,是一种在血液中循环的抗体,可以协助吞噬破坏外来微生物。它在抗体依赖性细胞介导的细胞毒性中发挥重要作用,并且还与II型和III型超敏反应有关。抗兔IgG(全分子)过氧化物酶偶联抗体可用于pAb-ELISA。兔抗IgG抗体与过氧化物酶(在TBS、0.5% BSA中以1:2500稀释)可用于蛋白质印迹。该抗体还用作间接ELISA法进行蛤蚌毒素分析的检测器。山羊抗兔IgG过氧化物酶缀合物与所有兔免疫球蛋白发生特异性反应。
免疫原
纯化兔 IgG。
应用
抗兔 IgG(全分子)过氧化物酶结合物抗体用于蛋白印迹法、间接 ELISA、免疫印迹和免疫检测。
用 HRP 标记的羊抗兔 IgG 作为二抗,对 C33A 细胞裂解物进行免疫共沉淀和免疫印迹分析。
用HRP标记的山羊抗兔IgG作为二抗,对小鼠肠、肝和脾的冰冻切片 (10um) 进行免疫组化分析。在与二抗孵育之前,用MeOH/过氧化氢30%的混合物处理切片,以阻断内源过氧化物酶。
外形
0.01 M磷酸盐缓冲液(pH 7.4,含有1%牛血清白蛋白和防腐剂)的溶液。
制备说明
按照Wilson, M.B.,和Nakane, P.K.在由阿姆斯特丹Elsevier/北荷兰生物医学出版社出版的免疫荧光及相关染色技术(1978)一书中所描述的高碘酸盐法而制备。
免责声明
除非我们的目录或随附于产品的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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警示用语:
Warning
危险分类
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
含少量动物源组分生物产品
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