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Merck
CN

A8275

抗-兔IgG(全分子)-过氧化物酶 山羊抗

IgG fraction of antiserum, buffered aqueous solution

别名:

Goat Anti-Rabbit IgG (whole molecule)–HRP

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UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
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产品名称

抗-兔IgG(全分子)-过氧化物酶 山羊抗, IgG fraction of antiserum, buffered aqueous solution

biological source

goat

conjugate

peroxidase conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:10,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

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Disclaimer

除非我们的目录或随附于产品的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

Application

抗兔 IgG(全分子)过氧化物酶结合物抗体用于蛋白印迹法、间接 ELISA、免疫印迹和免疫检测。
用 HRP 标记的羊抗兔 IgG 作为二抗,对 C33A 细胞裂解物进行免疫共沉淀和免疫印迹分析。
用HRP标记的山羊抗兔IgG作为二抗,对小鼠肠、肝和脾的冰冻切片 (10um) 进行免疫组化分析。在与二抗孵育之前,用MeOH/过氧化氢30%的混合物处理切片,以阻断内源过氧化物酶。

General description

IgG是一种单体,是一种在血液中循环的抗体,可以协助吞噬破坏外来微生物。它在抗体依赖性细胞介导的细胞毒性中发挥重要作用,并且还与II型和III型超敏反应有关。抗兔IgG(全分子)过氧化物酶偶联抗体可用于pAb-ELISA。兔抗IgG抗体与过氧化物酶(在TBS、0.5% BSA中以1:2500稀释)可用于蛋白质印迹。该抗体还用作间接ELISA法进行蛤蚌毒素分析的检测器。山羊抗兔IgG过氧化物酶缀合物与所有兔免疫球蛋白发生特异性反应。

Immunogen

纯化兔 IgG。

Physical form

0.01 M磷酸盐缓冲液(pH 7.4,含有1%牛血清白蛋白和防腐剂)的溶液。

Preparation Note

按照Wilson, M.B.,和Nakane, P.K.在由阿姆斯特丹Elsevier/北荷兰生物医学出版社出版的免疫荧光及相关染色技术(1978)一书中所描述的高碘酸盐法而制备。

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pictograms

Exclamation markEnvironment

signalword

Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

存储类别

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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Current Protocols in Immunology, doi: 10-doi: 10 (2001)
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