产品名称
抗-兔IgG(全分子)-过氧化物酶 山羊抗, IgG fraction of antiserum, buffered aqueous solution
biological source
goat
conjugate
peroxidase conjugate
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:10,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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Disclaimer
除非我们的目录或随附于产品的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Application
抗兔 IgG(全分子)过氧化物酶结合物抗体用于蛋白印迹法、间接 ELISA、免疫印迹和免疫检测。
用 HRP 标记的羊抗兔 IgG 作为二抗,对 C33A 细胞裂解物进行免疫共沉淀和免疫印迹分析。
用HRP标记的山羊抗兔IgG作为二抗,对小鼠肠、肝和脾的冰冻切片 (10um) 进行免疫组化分析。在与二抗孵育之前,用MeOH/过氧化氢30%的混合物处理切片,以阻断内源过氧化物酶。
General description
IgG是一种单体,是一种在血液中循环的抗体,可以协助吞噬破坏外来微生物。它在抗体依赖性细胞介导的细胞毒性中发挥重要作用,并且还与II型和III型超敏反应有关。抗兔IgG(全分子)过氧化物酶偶联抗体可用于pAb-ELISA。兔抗IgG抗体与过氧化物酶(在TBS、0.5% BSA中以1:2500稀释)可用于蛋白质印迹。该抗体还用作间接ELISA法进行蛤蚌毒素分析的检测器。山羊抗兔IgG过氧化物酶缀合物与所有兔免疫球蛋白发生特异性反应。
Immunogen
纯化兔 IgG。
Physical form
0.01 M磷酸盐缓冲液(pH 7.4,含有1%牛血清白蛋白和防腐剂)的溶液。
Preparation Note
按照Wilson, M.B.,和Nakane, P.K.在由阿姆斯特丹Elsevier/北荷兰生物医学出版社出版的免疫荧光及相关染色技术(1978)一书中所描述的高碘酸盐法而制备。
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Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
含少量动物源组分生物产品
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