product name
血纤维蛋白溶酶原激活物抑制剂1(PAI-1) 人, recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
重组
expressed in E. coli
质量水平
检测方案
≥90% (SDS-PAGE)
形式
lyophilized powder
比活
≥200,000 units/mg protein
分子量
~43 kDa
溶解性
water: 0.1 mL, clear, colorless
UniProt登记号
运输
dry ice
储存温度
−20°C
基因信息
human ... SERPINE1(5054)
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一般描述
纤溶酶原激活物抑制剂1 (PAI-1)或丝氨酸蛋白酶抑制剂E1 (SERPINE1)基因映射到人类染色体7q22.1。PAI-1在肝细胞、内皮细胞、平滑肌细胞、巨核细胞和脂肪细胞中产生。
应用
人纤溶酶原激活物抑制剂1 (PAI-1)已被用作：
- 放射免疫沉淀法(RIPA)中的脑源性神经营养因子(proBDNF)的抑制剂，用于外周血和淋巴细胞
- 阻断感染白色念珠菌菌丝
- 小鼠的纤溶酶活性，检测其对神经元神经突生长的影响
生化/生理作用
纤溶酶原激活物抑制剂1 (PAI -1)参与介导细胞衰老。它在损伤期间与其他组织修复转录组一起上调。PAI-1水平升高被认为是与心肌梗死(MI)相关的风险之一。
纤溶酶原激活物抑制剂1（PAI-1）蛋白属于丝氨酸蛋白酶抑制剂的Serpin超家族。它是组织纤溶酶原激活物（tPA）和尿激酶（uPA）的主要抑制剂，并也是纤溶酶原的激活剂，因此可抑制纤维蛋白溶解过程。 PAI可参与到细胞外基质的重塑，并涉及血管生成、趋化性、排卵和胚胎生成等过程。 PAI-1在各种疾病状态中的水平都有所上升，如多种癌症形式、肥胖症和代谢综合征。
单位定义
1单位可在生理状态下（中性pH和37 ºC）不可逆的抑制（100%抑制）1单位的组织纤溶酶原激活物（tPA）。
外形
以含有0.5 M氯化钠、20 mM 乙酸钠和50 mg/mL 海藻糖的一种溶液冻干粉形式提供。
重悬
用超纯水重悬成0.25 mg/ml。在0.5 M氯化钠、20 mM 乙酸钠和50 mg/mL 海藻糖中，含重悬产物0.25 mgP/mL。
分析说明
该产品是一种活性人PAI-1蛋白，分子量为43 kDa。
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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