生物来源
synthetic
质量水平
形式
emulsion
包含
emulsifier
技术
cell culture | hybridoma: suitable
microbiological culture: suitable
mp
0 °C (32 °F)
一般描述
消泡剂C乳液是含有30％活性硅的水性乳液。这种基于硅胶的消泡剂乳液含有不同于消泡剂乳液B和Y-30的非离子乳化剂。消泡剂C可以用3-10份冷水预稀释以帮助分散。预稀释后的悬浮液应立即使用。消泡剂C乳液通常在1-10 ppm时有效。消泡剂C乳液良好的流动性使得它可以根据需要泵入发酵罐，同时充分搅拌以分散消泡剂。消泡剂C乳液可用于控制哺乳动物细胞培养生物反应器顶部空间的起泡，最高使用浓度30 ppm。
应用
消泡剂C已用于：
- 添加到RO-H2O和化学成分确定培养基中，用于测定哺乳动物细胞培养物中的体积传质系数(kLa)
- 作为补料培养基组分，
- 作为生物控制器中的蒸汽灭菌消泡剂，用于pH-stat法分批补料培养
- 作为分批补料培养基补充剂，模拟典型的大规模培养条件
特点和优势
- 最大程度减少生物学系统的相容性问题
- 低浓度下的高效消泡剂
- 高效的多功能有机硅消泡剂
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Bernhard Sissolak et al.
Biotechnology journal, 14(7), e1800714-e1800714 (2019-04-16)
Frequently measured mammalian cell culture process indicators include viability and total cell concentration (TCC). Cell lysis, an additional important process characteristic that substantially contributes to the overall product purity profiles, is often not addressed in detail. In the present study
Gang Fu et al.
Biotechnology and applied biochemistry, 67(5), 812-818 (2019-10-08)
d-Psicose 3-epimerase is an enzyme that catalyzes the synthesis of d-psicose from d-fructose. We cloned the d-psicose 3-epimerase from Ruminococcus sp. (RDPE) and expressed it in Bacillus subtilis A311. By a two-step pH regulation of segmented fermentation, we significantly improved
Characterizing the Effects of Antifoam C Emulsion on Oxygen Mass Transfer within the BIOne Drilled-Hole Sparger and Microsparger Single-Use Bioreactor Systems
McAndrew J and Kauffman G
American Pharmacy null
Mervyn W O Liew et al.
Journal of biotechnology, 150(2), 224-231 (2010-08-28)
This study demonstrates the feasibility of large-scale production of murine polyomavirus VP1 protein in recombinant Escherichia coli as pentamers which are able to subsequently self-assemble in vitro into virus-like particles (VLPs). High-cell-density pH-stat fed-batch cultivation was employed to produce glutathione-S-transferase
Magdalena Pappenreiter et al.
Frontiers in bioengineering and biotechnology, 7, 195-195 (2019-09-10)
In aerobic cell cultivation processes, dissolved oxygen is a key process parameter, and an optimal oxygen supply has to be ensured for proper process performance. To achieve optimal growth and/or product formation, the rate of oxygen transfer has to be
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