推荐产品
生物来源
goat
质量水平
偶联物
alkaline phosphatase conjugate
抗体形式
affinity isolated antibody
抗体产品类型
secondary antibodies
克隆
polyclonal
表单
buffered aqueous glycerol solution
技术
direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20
western blot (chemiluminescent): 1:30,000 using β-actin in total cell extract of HiLa cells (5-10 μg per well)
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
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一般描述
Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The product does not react with mouse IgG, Fab fragment, human IgG, IgA, and IgM, or rat IgG.
特异性
Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The product does not react with mouse IgG, Fab fragment, human IgG, IgA, and IgM, or rat IgG.
免疫原
Purified mouse IgG, Fc fragment
应用
碱性磷酸酶缀合的山羊抗小鼠 Fc 特异性抗体在 ELISA 测定中用作二抗，在 PBS/0.1% Tween 和 1% BSA中以 1:1000 的稀释度，在 37°C 下持续 1.5 小时。该抗体经开发使用磷酸 4-硝基苯酯（Sigma）作为底物，在 37°C 下，持续 30分钟。
其他说明
Antibody adsorbed with human IgG and rat serum proteins.
外形
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide.
制备说明
Adsorbed to reduce background staining with human or rat samples.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
含少量动物源组分生物产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Pasquale Gallo et al.
International journal of cancer, 113(1), 67-77 (2004-09-24)
The protective efficacy of xenogeneic vaccination with DNA encoding the HER2 oncogene was evaluated in BALB/c mice transgenic for the transforming form of the neu oncogene, which spontaneously develops carcinomas in all mammary glands. Intramuscular injection of either plasmid DNA
Jeroen D Langereis et al.
Clinical & translational immunology, 10(4), e1256-e1256 (2021-04-13)
Complete deficiency of alternative pathway (AP) complement factors, explained by homozygous mutations, is a well-known risk factor for invasive bacterial infections; however, this is less obvious for heterozygous mutations. We describe two siblings with a heterozygous NM_001928.3(CFD):c.125C>A p.(Ser42*) mutation in
Maria Domina et al.
Scientific reports, 6, 31458-31458 (2016-08-18)
We have recently described a method, named PROFILER, for the identification of antigenic regions preferentially targeted by polyclonal antibody responses after vaccination. To test the ability of the technique to provide insights into the functional properties of monoclonal antibody (mAb)
Cyclin C directly stimulates Drp1 GTP affinity to mediate stress-induced mitochondrial hyperfission.
Vidyaramanan Ganesan et al.
Molecular biology of the cell, 30(3), 302-311 (2018-12-06)
Mitochondria exist in an equilibrium between fragmented and fused states that shifts heavily toward fission in response to cellular damage. Nuclear-to-cytoplasmic cyclin C relocalization is essential for dynamin-related protein 1 (Drp1)-dependent mitochondrial fission in response to oxidative stress. This study
A I Bot et al.
Pharmaceutical research, 17(3), 275-283 (2000-05-09)
Delivery of specific antibodies or immunoglobulin constructs to the respiratory tract may be useful for prophylaxis or active treatment of local or systemic disorders. Therefore, we evaluated the utility of lipid-based hollow-porous microparticles (PulmoSpheres) as a potential delivery vehicle for
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