产品名称
抗 小鼠 IgG（Fc 特异性）-碱性磷酸酶 山羊抗, affinity isolated antibody, buffered aqueous glycerol solution
biological source
goat
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
technique(s)
direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20
western blot (chemiluminescent): 1:30,000 using β-actin in total cell extract of HiLa cells (5-10 μg per well)
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
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Application
碱性磷酸酶缀合的山羊抗小鼠 Fc 特异性抗体在 ELISA 测定中用作二抗，在 PBS/0.1% Tween 和 1% BSA中以 1:1000 的稀释度，在 37°C 下持续 1.5 小时。该抗体经开发使用磷酸 4-硝基苯酯（Sigma）作为底物，在 37°C 下，持续 30分钟。
Biochem/physiol Actions
Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The product does not react with mouse IgG, Fab fragment, human IgG, IgA, and IgM, or rat IgG.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The product does not react with mouse IgG, Fab fragment, human IgG, IgA, and IgM, or rat IgG.
Immunogen
Purified mouse IgG, Fc fragment
Other Notes
Antibody adsorbed with human IgG and rat serum proteins.
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide.
Preparation Note
Adsorbed to reduce background staining with human or rat samples.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
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