推荐产品
生物来源
goat
质量水平
偶联物
peroxidase conjugate
抗体形式
affinity isolated antibody
抗体产品类型
secondary antibodies
克隆
polyclonal
表单
buffered aqueous solution
技术
direct ELISA: 1:10,000
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
一般描述
IgG由四个亚类组成,即IgG1,IgG2,IgG3和IgG4。IgG由四条多肽链组成-两条重链(γ链)和两条轻链(κ或λ链),它们通过链间二硫键连接。重链由N-末端可变域(VH)和三个恒定域(CH1,CH2,CH3)组成。在CH1和CH2区域之间存在铰链区域。轻链具有一个N-末端可变域(VL)和一个恒定域(CL)。
免疫原
纯化豚鼠IgG
应用
抗豚鼠IgG(全分子)-过氧化物酶标记抗体适用于免疫细胞化学和ELISA分析。
采用ELISA法,此HRP标记山羊抗豚鼠IgG(A7289)用1% BSA/PBS按1:2000稀释,在37°C反应1小时,检测豚鼠血清中抗幽门螺杆菌IgG。
生化/生理作用
IgG是糖蛋白抗体,可调控几种免疫应答。在母体通过胎盘向婴儿提供免疫力中IgG起主要作用。IgG可激活补体系统和调节抗体依赖性细胞介导的细胞毒性(ADCC)。
靶蛋白特异性的豚鼠IgG通常在多种研究应用中用作一抗。因此,标记可检测底物的抗豚鼠IgG二抗是分析靶蛋白的有用工具。抗豚鼠IgG(全分子)-过氧化物酶抗体对豚鼠IgG具有特异性。
外形
0.01 M磷酸盐缓冲液,pH 7.4,含有1%牛血清白蛋白和防腐剂。
制备说明
该抗血清以纯化的豚鼠IgG为免疫原在山羊体内免疫获得。通过免疫特异性纯化从山羊抗豚鼠IgG抗血清中分离抗体,该过程可基本上去除所有山羊血清蛋白,包括免疫球蛋白(其不可与豚鼠IgG特异性结合)。山羊抗豚鼠IgG通过Wilson和Nakane的过碘酸法标记上Sigma辣根过氧化物酶VI型(货号P8375)。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的人类或动物食用或应用。
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警示用语:
Warning
危险分类
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
含少量动物源组分生物产品
常规特殊物品
从最新的版本中选择一种:
分析证书(COA)
Infection and immunity, 66(6), 2614-2618 (1998-05-29)
Humans infected with Helicobacter pylori have abnormally low levels of the antioxidant vitamin C, which protects against the formation of carcinogenic nitrosamines, in gastric juice. Guinea pigs, like humans and nonhuman primates, have a dietary requirement for vitamin C. As
Scientific reports, 8(1), 11877-11877 (2018-08-23)
The European honeybee (Apis mellifera L.) exhibits various social behaviors. The molecular and neural mechanisms underlying these behaviors have long been explored, but causal relations between genes or neurons and behaviors remain to be elucidated because effective gene manipulation methods
PloS one, 10(3), e0115832-e0115832 (2015-03-31)
The Arabidopsis E-NTPDase (ecto-nucleoside triphosphate diphosphohydrolase) AtAPY1 was previously shown to be involved in growth and development, pollen germination and stress responses. It was proposed to perform these functions through regulation of extracellular ATP signals. However, a GFP-tagged version was
Gene therapy, 24(12), 757-767 (2017-11-07)
DNA vaccines delivered using electroporation (EP) have had clinical success, but these EP methods generally utilize invasive needle electrodes. Here, we demonstrate the delivery and immunogenicity of a DNA vaccine into subcutaneous adipose tissue cells using noninvasive EP. Using finite
Experimental & applied acarology, 13(2), 153-159 (1991-12-11)
Immunocytochemical staining based on the peroxidase-antiperoxidase method has shown that some neurosecretory cells (NSC) in the synganglion of the adult female tick Ornithodoros parkeri react with an antibody to bovine insulin. There are 18 regions of paraldehyde fuchsin-positive NSC of
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