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Merck
CN

A7130

β-淀粉酶 来源于大麦

greener alternative

Type II-B, 20-80 units/mg protein (biuret)

别名:

1,4-α-D-葡聚糖麦芽糖水解酶

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化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-566-1
MDL number:
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产品名称

β-淀粉酶 来源于大麦, Type II-B, 20-80 units/mg protein (biuret)

type

Type II-B

form

powder

specific activity

20-80 units/mg protein (biuret)

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

greener alternative category

shipped in

wet ice

storage temp.

2-8°C

Quality Level

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Analysis Note

粗品

Application

β-淀粉酶用于水解 α-连接的多糖(例如淀粉和糖原)的 α 键。β-淀粉酶已经用于各种植物研究,例如山杨的碳饥饿研究。来自大麦的 β-淀粉酶已用于研究压力和温度是如何影响催化活性的

Biochem/physiol Actions

β-淀粉酶水解三个或更多个 α-(1,4)连接的 D-葡萄糖单元的多糖中的 α-(1,4)葡聚糖键。淀粉和糖原等天然底物被分解成葡萄糖和麦芽糖。纯的结晶 β-淀粉酶制剂由具有不同等电点的四种同工酶组成。在不存在还原剂的情况下,酶通过巯基非常快速地聚合。对氯甲基苯甲酸酯抑制该聚合和该酶活性。还原剂巯基乙醇或二硫苏糖醇可以完全恢复该活性。

General description

我们致力于为您带来更加绿色的替代产品,这些产品遵守一项或多项绿色化学12项原则。该产品经过改进,可在用于淀粉乙醇研究时提高能效和防止浪费。有关详细信息,请参阅 《Biofiles》的文章

Other Notes

在 pH 4.8,温度 20 °C 条件下,一个单位可在 3 分钟内从淀粉中释放出 1.0 mg 麦芽糖。

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

动植物源性产品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Purification and characterisation of crystalline -amylase from barley.
K Visuri et al.
European journal of biochemistry, 28(4), 555-565 (1972-08-04)
Volker Heinz et al.
Biotechnology progress, 21(6), 1632-1638 (2005-12-03)
The depolymerization of starch by beta-amylase during exposure to hydrostatic pressure up to 700 MPa and within a temperature range from 20 to 70 degrees C has been investigated. Inactivation of the enzyme as well as alterations in conversion speed
William R L Anderegg et al.
Plant physiology, 159(4), 1866-1874 (2012-06-06)
Drought impacts on forests, including widespread die-off, are likely to increase with future climate change, although the physiological responses of trees to lethal drought are poorly understood. In particular, in situ examinations of carbon starvation and its interactions with and
Ling-Wei Meng et al.
Food science and biotechnology, 29(4), 503-512 (2020-04-17)
The rice flours were hydrolyzed using α-amylase (A), α-amylase and xylanase (AX), and α-amylase, xylanase and β-amylase (AXB). The effects of different enzymatic rice flour hydrolysates (ERH) on the quality of the fermented rice cake (FRC) were determined at 25 °C
Ambarish Nag et al.
BMC systems biology, 5, 94-94 (2011-06-21)
Higher plants and algae are able to fix atmospheric carbon dioxide through photosynthesis and store this fixed carbon in large quantities as starch, which can be hydrolyzed into sugars serving as feedstock for fermentation to biofuels and precursors. Rational engineering

实验方案

Beta-amylase is found in bacteria, fungi, and plants. To measure β-amylase activity, this assay uses a colorimetric spectrophotometric stop reaction at 540 nm.

β-淀粉酶存在于细菌、真菌和植物中。在测定β-淀粉酶活性时,该测定使用了一种在540nm处的比色分光光度终点反应法进行测定。

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