类型
Type II-B
质量水平
形式
powder
比活
20-80 units/mg protein (biuret)
环保替代产品特性
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
环保替代产品分类
, Enabling
运输
wet ice
储存温度
2-8°C
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一般描述
我们致力于为您带来更加绿色的替代产品，这些产品遵守一项或多项绿色化学12项原则。该产品经过改进，可在用于淀粉乙醇研究时提高能效和防止浪费。有关详细信息，请参阅 《Biofiles》的文章。
应用
β-淀粉酶用于水解 α-连接的多糖（例如淀粉和糖原）的 α 键。β-淀粉酶已经用于各种植物研究，例如山杨的碳饥饿研究。来自大麦的 β-淀粉酶已用于研究压力和温度是如何影响催化活性的。
生化/生理作用
β-淀粉酶水解三个或更多个 α-（1,4）连接的 D-葡萄糖单元的多糖中的 α-（1,4）葡聚糖键。淀粉和糖原等天然底物被分解成葡萄糖和麦芽糖。纯的结晶 β-淀粉酶制剂由具有不同等电点的四种同工酶组成。在不存在还原剂的情况下，酶通过巯基非常快速地聚合。对氯甲基苯甲酸酯抑制该聚合和该酶活性。还原剂巯基乙醇或二硫苏糖醇可以完全恢复该活性。
质量
粗品
单位定义
在 pH 4.8，温度 20 °C 条件下，一个单位可在 3 分钟内从淀粉中释放出 1.0 mg 麦芽糖。
其他说明
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
动植物源性产品
Volker Heinz et al.
Biotechnology progress, 21(6), 1632-1638 (2005-12-03)
The depolymerization of starch by beta-amylase during exposure to hydrostatic pressure up to 700 MPa and within a temperature range from 20 to 70 degrees C has been investigated. Inactivation of the enzyme as well as alterations in conversion speed
Purification and characterisation of crystalline -amylase from barley.
K Visuri et al.
European journal of biochemistry, 28(4), 555-565 (1972-08-04)
Jun Li et al.
Molecular plant-microbe interactions : MPMI, 24(10), 1165-1178 (2011-06-09)
Microbial volatiles promote the accumulation of exceptionally high levels of starch in leaves. Time-course analyses of starch accumulation in Arabidopsis leaves exposed to fungal volatiles (FV) emitted by Alternaria alternata revealed that a microbial volatile-induced starch accumulation process (MIVOISAP) is
Heike Reinhold et al.
The Plant cell, 23(4), 1391-1403 (2011-04-14)
Plants contain β-amylase-like proteins (BAMs; enzymes usually associated with starch breakdown) present in the nucleus rather than targeted to the chloroplast. They possess BRASSINAZOLE RESISTANT1 (BZR1)-type DNA binding domains--also found in transcription factors mediating brassinosteroid (BR) responses. The two Arabidopsis
Claudinéia Aparecida Soares et al.
Journal of agricultural and food chemistry, 59(12), 6672-6681 (2011-05-20)
Different banana cultivars were used to investigate the influences of starch granule structure and hydrolases on degradation. The highest degrees of starch degradation were observed in dessert bananas during ripening. Scanning electron microscopy images revealed smooth granule surface in the
实验方案
β-淀粉酶存在于细菌、真菌和植物中。在测定β-淀粉酶活性时，该测定使用了一种在540nm处的比色分光光度终点反应法进行测定。
Beta-amylase is found in bacteria, fungi, and plants. To measure β-amylase activity, this assay uses a colorimetric spectrophotometric stop reaction at 540 nm.
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