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Merck
CN

A7005

β-淀粉酶 来源于甘薯

greener alternative

Type I-B, ammonium sulfate suspension, ≥750 units/mg protein (E1%/280)

别名:

1,4-α-D-葡聚糖麦芽水解酶

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关于此项目

化学文摘社编号:
9000-91-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-566-1
MDL number:
MFCD00081391
EC 号:
3.2.1.2 (BRENDA, IUBMB)

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产品名称

β-淀粉酶 来源于甘薯, Type I-B, ammonium sulfate suspension, ≥750 units/mg protein (E1%/280)

Quality Level

200

biological source

sweet potato

type

Type I-B

form

ammonium sulfate suspension

specific activity

≥750 units/mg protein (E1%/280)

greener alternative product score

old score: 57
new score: 1
Find out more about DOZN™ Scoring

greener alternative product characteristics

Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

concentration

≥7.0 mg/mL

greener alternative category

, Re-engineered

storage temp.

2-8°C

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相关类别

Application

β -淀粉酶可用于水解 α-连接的多糖（例如淀粉和糖原）α连接键。 β Sigma的-淀粉酶可用于各种植物研究，如杨树的碳饥饿研究。β来源于甘薯的-淀粉酶可用于检查该酶对金黄色葡萄球菌生物膜的抑制和去除作用。该酶也可用于由糯玉米淀粉制备β-极限糊精。
β甘薯-淀粉酶可用于：
  • 水解淀粉和淀粉-果胶复合物
  • 比较金黄色葡萄球菌SH1000生物膜抑制和还原试验中不同来源的淀粉酶
  • 样品中的硒形态分析

Biochem/physiol Actions

β-淀粉酶可水解由三个或多个α-(1,4)连接D-葡萄糖单元组成的多糖的α-(1,4)葡聚糖键。天然底物（如淀粉和糖原）可被分解成葡萄糖和麦芽糖。β甘薯-淀粉酶是具有相同亚基的四聚体，其222个分子对称排列。
β-淀粉酶在检测糖苷酶之间的酶乱交中起重要作用。

General description

β-淀粉酶属于淀粉分解酶。
我们竭诚为您带来满足绿色替代产品四大类别要求的替代产品。本品属于重新设计产品类别，在“防止废弃物”、“更安全的溶剂和助剂”以及“节能设计”绿色化学原则方面取得了重大改进。 点击此处查看其 DOZN 记分卡。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。当用于淀粉乙醇研究时，该产品经优化可提高能效和防止浪费。有关详细信息，请参阅《Biofiles》的文章。

Other Notes

在 pH 4.8，温度 20 °C 条件下，一个单位可在 3 分钟内从淀粉中释放出 1.0 mg 麦芽糖。
更多关于复杂碳水化合物分析酶的信息请见www.sigma-aldrich.com/enzymeexplorer

Physical form

溶于2.3 M（NH4)2SO4的结晶混悬液

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

法规信息

动植物源性产品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000429463
0000429463
0000409467
0000409467
0000378834

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Mechanisms of enzymatic glycosyl transfer
Comprehensive Natural Products II (2010)
Effect of cross-linking on the resistance to enzymatic hydrolysis of waxy maize starch and low-methoxy pectin
Khondkar D, et al.
Food Hydrocolloids, 23(2), 387-393 (2009)
Xin Qi et al.
Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques, 14(1), 60-66 (2011-04-20)
β-Limit dextrin has been studied for many years as a means to investigate the internal structures of amylose and amylopectin. However its role as an excipient in the pharmaceutical industry has never been reported. This paper is the first one
C G Cheong et al.
Proteins, 21(2), 105-117 (1995-02-01)
Sweet potato beta-amylase is a tetramer of identical subunits, which are arranged to exhibit 222 molecular symmetry. Its subunit consists of 498 amino acid residues (Mr 55,880). It has been crystallized at room temperature using polyethylene glycol 1500 as precipitant.
William R L Anderegg et al.
Plant physiology, 159(4), 1866-1874 (2012-06-06)
Drought impacts on forests, including widespread die-off, are likely to increase with future climate change, although the physiological responses of trees to lethal drought are poorly understood. In particular, in situ examinations of carbon starvation and its interactions with and
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商品

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

如何使用溶于硫酸铵悬液的酶

以硫酸铵悬浮液形式提供的酶的使用指南

实验方案

Enzymatic Assay of β-AMYLASE (EC 3.2.1.2)

Beta-amylase is found in bacteria, fungi, and plants. To measure β-amylase activity, this assay uses a colorimetric spectrophotometric stop reaction at 540 nm.

β-淀粉酶(EC 3.2.1.2)的酶促检测

β-淀粉酶存在于细菌、真菌和植物中。在测定β-淀粉酶活性时，该测定使用了一种在540nm处的比色分光光度终点反应法进行测定。

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