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Merck
CN

A6664

Sigma-Aldrich

(4-脒苯基)甲磺酰氟盐酸盐 盐酸盐

serine protease inhibitor

别名:

p-APMSF, 对脒苯基甲磺酰氯盐酸盐 盐酸盐

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About This Item

经验公式(希尔记法):
C8H9FN2O2S · HCl
CAS号:
分子量:
252.69
Beilstein:
9083148
MDL编号:
UNSPSC代码:
12352202
PubChem化学物质编号:
NACRES:
NA.77

方案

≥97% (silver nitrate titration)

表单

powder

mp

190-191 ºC

溶解性

H2O: 50 mM (Stable when aliquoted at −20°C. Half-life = 6 minutes in pH 7.0 buffer systems.)

储存温度

−20°C

SMILES字符串

Cl[H].NC(=N)c1ccc(CS(F)(=O)=O)cc1

InChI

1S/C8H9FN2O2S.ClH/c9-14(12,13)5-6-1-3-7(4-2-6)8(10)11;/h1-4H,5H2,(H3,10,11);1H

InChI key

KHLLRHIUKOJXLL-UHFFFAOYSA-N

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一般描述

4-氨基苯甲磺酰氟盐酸盐相对无毒。

应用

4-氨基苯甲磺酰氟盐酸盐已用作:
  • 抑制剂鸡尾酒的组分,用于全血收集和血浆分离
  • 十二烷基硫酸钠(SDS)样品缓冲液的组分,用于制备全细胞提取物
  • 胰蛋白酶抑制剂,用于测量其抑制效率,并确定量子点荧光共振能量转移(FRET)式酶探针的功效

生化/生理作用

4-氨基苯甲磺酰氟盐酸盐(p-APMSF)能抑制镰孢菌产生的胰蛋白酶样蛋白酶。它还能够阻断牛Xa因子、人纤溶酶和人补体蛋白酶C1r和C1s。
具有赖氨酸或精氨酸底物特异性的丝氨酸蛋白酶的不可逆抑制剂。有效浓度是10-100 μM。常用于表征新发现的蛋白酶。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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分析证书(COA)

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Lifang Shi et al.
Analytical chemistry, 79(1), 208-214 (2006-12-30)
The paper describes the development and characterization of analytical properties of quantum dot-based probes for enzymatic activity and for screening enzyme inhibitors. The luminescent probes are based on fluorescence resonance energy transfer (FRET) between luminescent quantum dots that serve as
Anja I Pekkarinen et al.
Journal of agricultural and food chemistry, 50(13), 3849-3855 (2002-06-13)
The fungal disease Fusarium head blight occurs on wheat (Triticum spp.) and barley (Hordeum vulgare L.) and is one of the worldwide problems of agriculture. It can be caused by various Fusarium species. We are characterizing the proteinases of F.
R Laura et al.
Biochemistry, 19(21), 4859-4864 (1980-10-14)
p-(Amidinophenyl)methanesulfonyl fluoride (p-APMSF) has been synthesized and shown to be a specific, irreversible inhibitor of the class of plasma serine proteases which demonstrate substrate specificity for the positively charged side chains of the amino acid lysine or arginine. In equimolar
Yoshihiro Hayashi et al.
Cancer discovery, 8(11), 1438-1457 (2018-08-25)
Myelodysplastic syndromes (MDS) are heterogeneous hematopoietic disorders that are incurable with conventional therapy. Their incidence is increasing with global population aging. Although many genetic, epigenetic, splicing, and metabolic aberrations have been identified in patients with MDS, their clinical features are
Lacramioara Ivanciu et al.
Blood, 124(11), 1705-1714 (2014-05-30)
The membrane-dependent interaction of factor Xa (FXa) with factor Va (FVa) forms prothrombinase and drives thrombin formation essential for hemostasis. Activated platelets are considered to provide the primary biological surface to support prothrombinase function. However, the question of how other

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