产品名称
Anti-Mouse IgG (whole molecule)−Agarose antibody produced in goat, affinity isolated antibody, suspension
biological source
goat
conjugate
agarose conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
suspension
species reactivity
mouse
extent of labeling
≥5 mg per mL
technique(s)
Ouchterlony double diffusion: suitable
immunoprecipitation (IP): suitable
matrix
Cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
matrix spacer
1 atom
capacity
0.4 mg/mL, resin binding capacity (mouse IgG)
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunoprecipitation (1 paper)
Chromatin immunoprecipitation (1 paper)
Immunoprecipitation (1 paper)
Goat Anti-Mouse IgG (whole molecule)-Agarose antibodies have been used for immunoprecipiation assays. The product can also be used for Ouchterlony double diffusion applications.
Soluble antigens from meningococci extracts were mixed with ascitic fluid containing primary antibody and 50 μl of agarose-linked goat anti-mouse IgG for 5 hours at 4 °C.
Biochem/physiol Actions
IgG helps in opsonization, complement fixation and antibody-dependent cell-mediated cytotoxicity. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. IgG2a of IgG has cytotropic properties and IgG2 molecules have the ability to activate complement.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mice consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Goat Anti-Mouse IgG (whole molecule)-Agarose antibodies bind to all mouse IgG subclasses.
Immunogen
IgG isolated from pooled normal mouse serum
Physical form
Suspension in 0.5 M NaCl containing preservative
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存储类别
10 - Combustible liquids
wgk
WGK 3
法规信息
常规特殊物品
此项目有
C R Tinsley et al.
Journal of general microbiology, 138(11), 2321-2328 (1992-11-01)
The neisserial Lip antigen is a conserved antigen associated with the pathogenic Neisseria species, and is composed of multiple repeats of a consensus pentapeptide. A series of monoclonal antibodies reacting with meningococcal Lip antigen were subjected to epitope mapping, using
Riva Kovjazin et al.
PloS one, 9(1), e85400-e85400 (2014-01-15)
The MUC1 tumor associated antigen is highly expressed on a range of tumors. Its broad distribution on primary tumors and metastases renders it an attractive target for immunotherapy. After synthesis MUC1 is cleaved, yielding a large soluble extracellular alpha subunit
Alvaro Román-Fernández et al.
Science advances, 9(5), eabq1858-eabq1858 (2023-02-04)
The glycocalyx component and sialomucin podocalyxin (PODXL) is required for normal tissue development by promoting apical membranes to form between cells, triggering lumen formation. Elevated PODXL expression is also associated with metastasis and poor clinical outcome in multiple tumor types.
Yael Kalma et al.
Endocrinology, 145(4), 1617-1624 (2003-12-20)
The coordinated function of the different compartments of the follicle, the oocyte and the somatic cumulus/granulosa cells, is enabled by the presence of a network of cell-to-cell communication generated by gap junctions. Connexin 43 (Cx43) is the most abundant gap
Sara Landeras-Bueno et al.
PLoS pathogens, 7(11), e1002397-e1002397 (2011-11-25)
The influenza A virus RNA polymerase is a heterotrimeric complex responsible for viral genome transcription and replication in the nucleus of infected cells. We recently carried out a proteomic analysis of purified polymerase expressed in human cells and identified a
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