一般描述
IgG是糖蛋白抗体，可调控几种免疫应答。靶蛋白的兔IgG通常在多种研究应用中用作一抗。因此，与可检测底物缀合的抗兔IgG二抗是靶蛋白分析的有用工具。山羊抗兔IgG（全分子）-过氧化物酶抗体在缀合前通过与正常血清和兔IgG免疫电泳对兔IgG产生免疫特异性。
免疫原
纯化的兔IgG
应用
使用HRP标记的山羊抗兔IgG作为二抗，通过蛋白印迹分析C33A细胞裂解物。 对来自结肠粘膜提取物(μg)的蛋白等分试样进行蛋白印迹分析：使用HRP标记的山羊抗兔IgG作为二抗，用TBST以1:5000稀释，室温孵育1小时。
山羊抗兔IgG（全分子）-过氧化物酶抗体用于蛋白印迹（1:6,000）和ELISA检测。
用 HRP 标记的羊抗兔 IgG 作为二抗，对 C33A 细胞裂解物进行免疫共沉淀和免疫印迹分析。
外形
0.01 M磷酸盐缓冲液（pH 7.4，含有1％牛血清白蛋白和防腐剂）溶液。
制备说明
使用Wilson, M.B.和Nakane, P.K.在Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978)中描述的高碘酸盐法制备。
其他说明
免责声明
除非我们的产品目录或产品随附的其他公司文档中另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人或动物。
Not finding the right product?
Try our 产品选型工具.
警示用语：
Warning
危险分类
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
含少量动物源组分生物产品
常规特殊物品
Johan de Vogel et al.
Carcinogenesis, 29(2), 398-403 (2008-01-05)
Epidemiological and animal model studies suggest that a high intake of heme, present in red meat, is associated with an increased risk of colon cancer. The aim of this study was to elucidate the effects of dietary heme on colonic
Patrick Eldin et al.
Nucleic acids research, 42(3), 1698-1710 (2013-11-02)
The human immunodeficiency virus type 1 (HIV-1) Vpr protein binds to the cellular uracil-DNA glycosylase UNG2 and induces its degradation through the assembly with the DDB1-CUL4 ubiquitin ligase complex. This interaction counteracts the antiviral activity exerted by UNG2 on HIV-1
Sandra V Ashton et al.
Arteriosclerosis, thrombosis, and vascular biology, 25(1), 102-108 (2004-10-23)
Invasion of uterine spiral arteries by extravillous trophoblasts in the first trimester of pregnancy results in loss of endothelial and musculoelastic layers. This remodeling is crucial for an adequate blood supply to the fetus with a failure to remodel implicated
A conformational contribution of the luteinizing hormone-receptor ectodomain to receptor activation.
Pascal Nurwakagari et al.
Journal of molecular endocrinology, 38(1-2), 259-275 (2007-02-13)
Glycoprotein hormone receptors such as the lutropin/chorionic gonadotropin receptor (LHR) are characterized by a large N-terminal ectodomain (ECD), which is responsible for hormone-receptor interactions. For the closely related TSH receptor (TSHR), it has been proposed that the ECD also serves
A Moles et al.
Oncogene, 35(35), 4623-4632 (2016-02-09)
Post-translational modifications of nuclear factor (NF)-κB subunits provide a mechanism to differentially regulate their activity in response to the many stimuli that induce this pathway. However, the physiological significance of these modifications is largely unknown, and it remains unclear if
商品
Review the key factors that should figure in your decision to choose a secondary antibody. Learn about species, subclass, isotype, label, and more.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门