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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
ascites fluid
抗体产品类型
primary antibodies
克隆
AC-15, monoclonal
分子量
antigen 42 kDa
包含
15 mM sodium azide
种属反应性
sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig
请勿与下列物质发生反应
Dictyostelium discoideum
技术
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:1,000-1:2,000 using cultured human or chicken fibroblasts
western blot: 1:5,000-1:10,000 using cultured human or chicken fibroblast cell extracts
同位素/亚型
IgG1
UniProt登记号
应用
research pathology
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)
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一般描述
特异性
免疫原
应用
- 免疫荧光染色
- 免疫印迹
- 免疫组织化学
- 用作蛋白芯片的对照品
生化/生理作用
外形
其他说明
免责声明
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
从最新的版本中选择一种：
分析证书(COA)
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
What is the molecular weight of Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse?
Beta Actin has a molecular weight of 42 kDa.
Can I use Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse, to detect mouse beta actin?
This antibody will detect mouse beta actin. When used for western blotting, the secondary antibody may react with mouse IgG from the sample resulting with a bands at 50 kDa and 25 kDa (reducing conditions) or approximately 150 kDa (non-reducing conditions). It is best to use a directly conjugated antibody for immunohistochemistry or immunofluorescent assays. We offer Product numbers A3854, peroxidase conjugated.
What IgG light chain (kappa or lambda) is associated with Clone AC-15 for Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse?
We have not determined the light chain used by monoclonal antibody AC-15. 95% of mouse antibodies have kappa light chains.
Why do I have 3 bands on my western blot when using Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse?
While actin should run as a 42 kDa band when detected by Western blotting, there are recent observations that cleavage products can be seen in cells undergoing apoptosis. Initial cleavage gives a band at 41 kDa, which may appear as a doublet on the blot. In addition, further cleavage results in a major band at 30 kDa and a small band at 14 kDa which have been shown to be cleavage products due to the action of interleukin 1 -converting enzyme. This may explain the detection of smaller bands when blotting with this antibody. This banding pattern may be detected with other actin antibodies as well. Kayalar, C., et al., Proc. Natl. Acad. Sci., USA, 93:2234-2238, (1996).
What is the antibody concentration of Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse?
This antibody is ascities fluid. The amount of protein present and the amount of IgG1 present is indicated on the Lot-specific Certificate of Analysis.
What peroxidase-conjugated secondary antibody should I use with Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse?
We recommend using Product No. A9044, rabbit anti-mouse IgG, peroxidase conjugate as a secondary antibody. We also offer this same antibody directly conjugated to peroxidase as Product No. A3854.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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实验方案
Western blot protocol details protein transfer from gels to nitrocellulose, crucial for immunoblotting procedures in research.
带有用于不同印迹过程工作流程步骤的Western blot实验方法，描述了蛋白从SDS聚丙烯酰胺凝胶(SDS-PAGE)到硝酸纤维素膜(NC膜)的电泳转移。也称为免疫印迹实验方法。
Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.
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