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安全信息

A4596

Millipore

ANTI-FLAG® M1琼脂糖亲和凝胶

别名:

抗-ddddk, 抗-dykddddk

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.32

偶联物

agarose conjugate

质量水平

200

抗体产品类型

primary antibodies

表单

suspension

同位素/亚型

IgG12b

容量

≥0.6 mg/mL, gel binding capacity

储存温度

−20°C

相关类别

一般描述

ANTI-FLAG®M1琼脂亲和凝胶是一种纯化小鼠IgG2B单克隆抗体共价连接到琼脂糖上。

特异性

结合特异性：FLAG 序列
N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C自由N末端

应用

用于纯化N末端FLAG融合蛋白。由于结合过程不依赖Ca2+，蛋白质能采用含EDTA的缓冲液洗脱，也可以使用FLAG多肽或甘油-HLC缓冲液(pH3)通过标准方法洗脱。ANTI-FLAG M1不会和Met-FLAG融合蛋白结合，所以这种树脂不适合纯化未加工处理的细胞质表达融合蛋白。

该亲和凝胶可用于N末端FLAG融合蛋白的钙介导纯化。

免疫沉淀(IP)：适用

洗脱方法：FLAG多肽、甘氨酸、pH3.5 EDTA

更多产品信息请访问我们的FLAG®应用页面。

特点和优势

  • 通常从粗裂解物中纯化融合蛋白，然后只使用一步层析，纯化成单条带
  • 用EDTA温和洗脱，将融合蛋白从亲和树脂上洗脱下来。
  • FLAG多肽溶液可以用于FLAG融合蛋白温和地，非变性洗脱。

外形

球形琼脂糖悬浮在含10 mM磷酸钠、150mM NaCl (pH7.4)、0.02% (w/v) 叠氮化钠的50%甘油溶液中

其他说明

ANTI-FLAG® M1并不会和Met-FLAG融合蛋白结合，所以这种树脂不适合纯化未处理的细胞质表达融合蛋白。

法律信息

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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说明
价格

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
0000372421
0000370795
0000372422
0000372420
0000372422

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如果您需要特殊版本，可通过批号或批次号查找具体证书。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Will Product A4596, ANTI-FLAG® M1 Agarose Affinity Gel, work for my C-terminal FLAG-tagged protein?

    No.  The M1 FLAG antibody resin will only bind proteins that are free N-terminal FLAG tagged proteins.  It will not recognize C-terminal FLAG tagged proteins or Met-N-terminal FLAG tagged proteins.

  4. Can I use Phosphate Buffered Saline (PBS) instead of Tris Buffered Saline (TBS) with Product A4596, ANTI-FLAG® M1 Agarose Affinity Gel?

    We do not recommend the use of phosphate buffers since calcium is required for binding.  This can result in the formation of insoluble calcium phosphates in the presence of PBS.

  5. How much calcium is required for binding of my FLAG-tagged protein to Product A4596, ANTI-FLAG® M1 Agarose Affinity Gel?

    10 mM calcium chloride is recommended for binding.

  6. What reagents are compatible with Product A4596, ANTI-FLAG® M1 Agarose Affinity Gel?

    The ANTI-FLAG M1 Affinity Gel is resistant to the following detergents: 5.0 % Tween-20; 5.0 % Triton X-100; 0.1 % NP-40; 0.1 % CHAPS and 0.2 % digitonin. It can also be used with 1.0 M NaCl or 1.0 M urea. Do not use the gel in the presence of SDS, deoxycholate or guanidine HCl.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Phosphoribosylformylglycinamidine synthase (PFAS) is an essential enzyme in de novo synthesis of purine. Previously, PFAS has been reported to modulate RIG-I activation during viral infection via deamidation. In this study, we sought to identify potential substrates that PFAS can deamidate.
Bo Zhou et al.
Journal of molecular endocrinology, 55(3), 231-243 (2015-09-17)
Progranulin (PGRN) has recently emerged as an important regulator for insulin resistance. However, the direct effect of PGRN in vivo and the underlying role of progranulin in adipose insulin resistance involving the autophagy mechanism is not fully understood. In this
E Di Zazzo et al.
TheScientificWorldJournal, 2014, 565839-565839 (2014-08-13)
Recent studies have shown that hyperinsulinemia may increase the cancer risk. Moreover, many tumors demonstrate an increased activation of IR signaling pathways. Phosphatidylinositol 3-kinase (PI3K) is necessary for insulin action. In epithelial cells, which do not express GLUT4 and gluconeogenic
Cui-Jun Zhang et al.
The EMBO journal, 32(8), 1128-1140 (2013-03-26)
DNA methylation in transposons and other DNA repeats is conserved in plants as well as in animals. In Arabidopsis thaliana, an RNA-directed DNA methylation (RdDM) pathway directs de novo DNA methylation. We performed a forward genetic screen for suppressors of
Ayswarya Ravi et al.
International journal of molecular sciences, 19(2) (2018-02-22)
Correct folding and post-translational modifications are vital for therapeutic proteins to elicit their biological functions. Osteopontin (OPN), a bone regenerative protein present in a range of mammalian cells, is an acidic phosphoprotein with multiple potential phosphorylation sites. In this study
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