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Merck
CN

A3937

Sigma-Aldrich

抗-兔IgG(全分子),F(ab′)2 片段−碱性磷酸酶 山羊抗

affinity isolated antibody, buffered aqueous glycerol solution

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About This Item

MDL编号:
UNSPSC代码:
12352203
NACRES:
NA.46

生物来源

goat

质量水平

偶联物

alkaline phosphatase conjugate

抗体形式

affinity isolated antibody

抗体产品类型

secondary antibodies

克隆

polyclonal

表单

buffered aqueous glycerol solution

种属反应性

rabbit

技术

direct ELISA: 1:30,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000

运输

wet ice

储存温度

2-8°C

靶向翻译后修饰

unmodified

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一般描述

IgG是糖蛋白抗体,可调控几种免疫应答。靶蛋白的兔IgG通常在多种研究应用中用作一抗。因此,与可检测底物缀合的抗兔IgG二抗是靶蛋白分析的有用工具。抗兔IgG(全分子),(F(ab′)2)片段-碱性磷酸酶抗体结合全部兔免疫球蛋白。

免疫原

纯化兔 IgG

应用

以碱性磷酸酶标记的山羊抗兔F2片段特异性抗体为二抗,在室温下对前列腺上皮细胞裂解液(1:7500稀释)进行2小时蛋白质印迹分析。
成功使用该抗体的应用以及相关同行评审论文如下所示。
酶联免疫吸附试验免疫印迹法(1 篇)
蛋白印迹法(1 篇)
抗兔IgG(全分子),(F(ab′)2)片段-碱性磷酸酶抗体适用于免疫印迹(5μL)和直接ELISA(1:30,000)。该产品也可用于免疫组织化学(1:50,使用福尔马林进行固定、石蜡包埋切片)。

外形

溶剂为0.05 M Tris缓冲液,pH 8.0,含1 mM MgCl2、10 mM甘氨酸、1%牛血清白蛋白、50%甘油和15 mM叠氮化钠

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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