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Merck
CN

A3437

Sigma-Aldrich

抗-人 IgM(μ-链特异性)−碱性磷酸酶 山羊抗

affinity isolated antibody, buffered aqueous glycerol solution

别名:

Goat Anti-Human IgM (μ-chain specific)−AP

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About This Item

MDL编号:
UNSPSC代码:
12352203
NACRES:
NA.46

生物来源

goat

质量水平

偶联物

alkaline phosphatase conjugate

抗体形式

affinity isolated antibody

抗体产品类型

secondary antibodies

克隆

polyclonal

形式

buffered aqueous glycerol solution

种属反应性

human

技术

direct ELISA: 1:30,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000

运输

wet ice

储存温度

2-8°C

靶向翻译后修饰

unmodified

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相关类别

一般描述

免疫球蛋白M(IgM)抗体出现在感染过程的早期。 IgM抗体负责错配输血中的红细胞凝集。IgM的水平可能随疾病或感染的状况而变化。
碱性磷酸酶是可以催化对硝基苯磷酸(PNPP)等显色底物转化的酶;分别将发光底物(如CDP-Star®和荧光底物(如4-甲基伞形酮磷酸酯/4-MUP)转化成为可检测色素、发光剂或荧光剂。
在应答外部抗原第一次刺激时,人IgM是血液中最早出现的免疫球蛋白亚型。这类免疫球蛋白参与中枢神经系统(CNS)髓鞘修复。分泌型IgM水平升高与鼻结膜炎有关。当针对人IgA、IgG、IgM、本周蛋白κ和λ骨髓瘤蛋白进行测试时,抗人IgM(μ-链特异性)-碱性磷酸酶抗体对人IgM具有特异性。

免疫原

人IgM

应用

IgM是一种糖蛋白,重链上具有5个n连接的糖基化位点。可通过ELISA分析确定结合凝集素的IgM的糖基化形式,其中碱性磷酸酶标记的山羊抗人IgM可作为二抗(1:2000稀释),以对硝基苯基为底物(Sigma)。
山羊抗人IgM(μ-链特异性异性)-碱性磷酸酶抗体可用于斑点杂交和蛋白质免疫印迹分析(稀释度1:30,000)。
山羊抗人IgM(μ-链特异性)-碱性磷酸酶多克隆抗体可用于通过显色、化学发光和荧光免疫化学及免疫组织化学技术检测和定量人血清和生物体液中的IgM水平。
抗人IgM(μ-链特异性)-碱性磷酸酶抗体适用于ELISA。

外形

溶剂为0.05 M Tris缓冲液,pH 8.0,含1 mM MgCl2、10 mM甘氨酸、1%牛血清白蛋白、50%甘油和15 mM叠氮化钠

法律信息

CDP-Star is a registered trademark of Tropix, Inc.

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的人类或动物食用或应用。

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WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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