推荐产品
生物来源
goat
质量水平
偶联物
alkaline phosphatase conjugate
抗体形式
affinity isolated antibody
抗体产品类型
secondary antibodies
克隆
polyclonal
形式
buffered aqueous glycerol solution
种属反应性
human
技术
direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
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相关类别
一般描述
人IgG是糖蛋白抗体，含有两条相等的轻链和一对相同的重链。 IgG具有四种不同亚型，范围从IgG1到IgG4。这些抗体可调节机体对过敏和病原性感染的免疫反应。IgG还与补体结合和自身免疫疾病有关。经测试证明，抗人IgM（γ-链特异性）-碱性磷酸酶抗体针对人IgA、IgG、IgM、本周蛋白κ和λ骨髓瘤蛋白具有特异性。
免疫原
纯化人IgG
应用
将从大肠杆菌（ E. coli ）培养物中收集的蛋白质上样至SDS-page凝胶进行蛋白质免疫印迹分析，使用浓度为1:10000的碱性磷酸酶标记山羊抗人IgG（链特异性）作为二抗。使用按1:5000稀释于0.25% BSA/PBS中的碱性磷酸酶标记山羊抗人IgG（γ链特异性）抗体，通过Elisa法检测人血清中的IgG。使用对硝基苯磷酸底物(1mg/ml，Sigma)进行显色。
抗人IgG（γ-链特异性）-碱性磷酸酶抗体适用于ELISA检测（稀释度为1:2500和1:10,000）。该抗体还能用于蛋白质免疫印迹 （1:30,000）和免疫组织化学分析（使用甲醛固定石蜡包埋切片时，稀释度为1:50）。
外形
溶剂为0.05 M Tris缓冲液，pH 8.0，含1 mM MgCl2、10 mM甘氨酸、1%牛血清白蛋白、50%甘油和15 mM叠氮化钠
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
含少量动物源组分生物产品
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