A3150
抗人 IgG（γ-链特异性）-碱性磷酸酶 山羊抗
affinity isolated antibody, buffered aqueous glycerol solution
别名:
Goat Anti-Human IgG (γ-chain specific)−AP
产品名称
抗人 IgG（γ-链特异性）-碱性磷酸酶 山羊抗, affinity isolated antibody, buffered aqueous glycerol solution
biological source
goat
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
species reactivity
human
technique(s)
direct ELISA: 1:7,000-1:21,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
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Application
将从大肠杆菌（ E. coli ）培养物中收集的蛋白质上样至SDS-page凝胶进行蛋白质免疫印迹分析，使用浓度为1:10000的碱性磷酸酶标记山羊抗人IgG（链特异性）作为二抗。使用按1:5000稀释于0.25% BSA/PBS中的碱性磷酸酶标记山羊抗人IgG（γ链特异性）抗体，通过Elisa法检测人血清中的IgG。使用对硝基苯磷酸底物(1mg/ml，Sigma)进行显色。
将从大肠杆菌（ E. coli ）培养物中收集的蛋白质上样至SDS-page凝胶进行蛋白质免疫印迹分析，使用浓度为1:10000的碱性磷酸酶标记山羊抗人IgG（链特异性）作为二抗。使用BCIP/NBT底物(Sigma)，使膜显色。
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Enzyme-linked immunosorbent assay (1 paper)
Goat anti-human IgG (gamma-chain specific)-alkaline phosphatase antibody can be used for ELISA applications using serum samples from allergic and borderline hypertensive patients.
Biochem/physiol Actions
Human IgGs regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. Goat anti-human IgG (gamma-chain specific)-alkaline phosphatase antibody is specific for human IgG when tested against human IgA, IgG, IgM, Bence Jones Kappa, and Lambda myeloma proteins.
IgG1 is most abundant and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions. IgG4 is associated with asymptomatic infection. IgG antibody have enormous therapeutic potential and the Fc region aids in the development of therapeutic antibody.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Immunoglobulins (Igs) belongs to the immunoglobulin super-family. Each immunoglobin has two heavy (H) and two light (L) chains held together by disulfide linkages. Each light chain comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. IgG is a glycoprotein antibody that contains two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4.
Immunogen
purified human IgG
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 15 mM sodium azide and 50% glycerol
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存储类别
10 - Combustible liquids
wgk
WGK 2
法规信息
常规特殊物品
含少量动物源组分生物产品
此项目有
X Baur et al.
Allergy, 57(10), 943-945 (2002-09-25)
Although fungal phytase is frequently used as an additive to animal feed few investigations of its allergenic property have been conducted. Fifty-three subjects occupationally exposed to powdered phytase from Aspergillus niger were studied. Exposure data and symptoms were registered by
P Hühn et al.
Nucleic acids research, 25(2), 410-416 (1997-01-15)
During the analysis of the La (SS-B) autoantigen for catalytic activities an ATP-dependent double-stranded RNA unwinding activity was detected. Both native and recombinant La proteins from different species displayed this activity, which could be inhibited by monospecific anti-La antibodies. La
H Tröster et al.
Journal of autoimmunity, 8(6), 825-842 (1995-12-01)
Immunization of Balb/c mice with a homogeneously purified recombinant human La/SS-B protein resulted in activation of an autoreactive B cell secreting a novel monoclonal anti-La antibody termed La4B6. La4B6 reacted with La protein from a variety of sources including human
K P Ng et al.
Arthritis and rheumatism, 55(6), 900-904 (2006-12-02)
To identify the prevalence of serologically active clinically quiescent (SACQ) patients in a cohort of 290 patients with systemic lupus erythematosus (SLE). We investigated if the presence of anti-double-stranded DNA (anti-dsDNA) or antinucleosome (anti-NCS) antibodies during the SACQ period was
R Wu et al.
Hypertension (Dallas, Tex. : 1979), 33(1), 53-59 (1999-02-04)
-Elevated antibody levels to oxidized low-density lipoprotein (aOxLDL) have been shown to correlate with the degree of atherosclerosis in some studies. On the other hand, immunization of experimental animals with OxLDL, leading to enhanced aOxLDL levels, inhibits the development of
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Instructions
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