推荐产品
生物来源
mouse
质量水平
偶联物
alkaline phosphatase conjugate
抗体形式
purified immunoglobulin
抗体产品类型
secondary antibodies
克隆
RG-96, monoclonal
表单
buffered aqueous glycerol solution
种属反应性
rabbit
请勿与下列物质发生反应
goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken
技术
direct ELISA: 1:50,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
western blot: 1:200,000-1:400,000 using total cell extract of HeLa cells
同位素/亚型
IgG1
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
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一般描述
Monoclonal Anti-Rabbit IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is a major class of immunoglobulin. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE.
The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.
应用
Monoclonal Anti-Rabbit IgG (γ-chain specific)Alkaline Phosphatase antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.
生化/生理作用
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
外形
Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 2
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
含少量动物源组分生物产品
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Regulation of the megakaryocytic glycoprotein IX promoter by the oncogenic Ets transcription factor Fli-1
Bastian LS, et al.
Blood, 93(8), 2637-2644 (1999)
Tae-Geum Kim et al.
Molecular biotechnology, 28(1), 33-40 (2004-10-01)
A deoxyribonucleic acid (DNA) fragment encoding the cholera toxin B subunit (CTB) was linked 5' to the simian immunodeficiency virus (SIVmac) Gag p27 capsid gene (CTB-Gag). The fusion gene was transferred into Solanum tuberosum cells by Agrobacterium tumefaciens-mediated transformation methods
Factors determining the density of AQP4 water channel molecules at the brain-blood interface
Hoddevik EH, et al.
Brain Structure &Amp; Function, 222(4), 1753-1766 (2017)
Interaction of Mannan Binding Lectin with alpha2 Macroglobulin via Exposed Oligomannose Glycans A CONSERVED FEATURE OF THE THIOL ESTER PROTEIN FAMILY
Arnold JN, et al.
The Journal of Biological Chemistry, 281(11), 6955-6963 (2006)
Heidi C Schmid et al.
Biological chemistry, 383(11), 1769-1778 (2003-01-18)
The reaction of recombinant chlorophyll synthase from Avena sativa, expressed in Escherichia coli, was investigated. To verify the identity of the recombinant and native enzymes, reaction rates were determined for both enzyme preparations with several chlorophyllide analogs. The rates of
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