推荐产品
生物来源
goat
质量水平
偶联物
alkaline phosphatase conjugate
抗体形式
affinity isolated antibody
抗体产品类型
secondary antibodies
克隆
polyclonal
表单
buffered aqueous solution
种属反应性
mouse
请勿与下列物质发生反应
bovine, human, horse
技术
direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
western blot (chemiluminescent): 1:160,000-1:320,000
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
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一般描述
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG1 regulates complement fixation in mice.
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
免疫原
Purified mouse IgG, Fc fragment
应用
碱性磷酸酶缀合的山羊抗小鼠 Fc 特异性抗体在 ELISA 测定中用作二抗，在 PBS/0.1% Tween 和 1% BSA中以 1:1000 的稀释度，在 37°C 下持续 1.5 小时。该抗体经开发使用磷酸 4-硝基苯酯（Sigma）作为底物，在 37°C 下，持续 30分钟。
Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is suitable for use in ELISA (1:1500) and western blot. The antibody can also be used for immunohistochemistry (1:40 using formalin-fixed, paraffin-embedded sections).
Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody produced in goat has been used in enzyme-linked immunosorbent assay (ELISA), sandwich ELISA and enzyme-linked immune absorbent spot (ELISPOT).
The product has been used as a secondary antibody for detecting mouse serum anti-hemagglutinin (HA) antibodies using ELISA techniques.
生化/生理作用
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
其他说明
Antibody adsorbed with bovine, equine and human serum proteins.
外形
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.
制备说明
Adsorbed to reduce background staining with bovine, horse, or human samples.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
含少量动物源组分生物产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Pedro Humberto Castro et al.
Journal of experimental botany, 69(19), 4633-4649 (2018-07-28)
Post-translational modifiers such as the small ubiquitin-like modifier (SUMO) peptide act as fast and reversible protein regulators. Functional characterization of the sumoylation machinery has determined the key regulatory role that SUMO plays in plant development. Unlike components of the SUMO
Release of Staphylococcus aureus extracellular vesicles and their application as a vaccine platform.
Xiaogang Wang et al.
Nature communications, 9(1), 1379-1379 (2018-04-13)
Secretion of extracellular vesicles (EVs), a process common to eukaryotes, archae, and bacteria, represents a secretory pathway that allows cell-free intercellular communication. Microbial EVs package diverse proteins and influence the host-pathogen interaction, but the mechanisms underlying EV production in Gram-positive
Antibody structure, instability, and formulation
Wang W, et al.
Journal of Pharmaceutical Sciences, 91(6), 252-265 (2007)
The specificity of targeted vaccines for APC surface molecules influences the immune response phenotype
Grodeland G, et al.
Testing, 8(11), e80008-e80008 (2013)
Rathi Prasad et al.
The Journal of clinical investigation, 127(3), 942-953 (2017-02-07)
Primary adrenal insufficiency is life threatening and can present alone or in combination with other comorbidities. Here, we have described a primary adrenal insufficiency syndrome and steroid-resistant nephrotic syndrome caused by loss-of-function mutations in sphingosine-1-phosphate lyase (SGPL1). SGPL1 executes the
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