推荐产品
应用
推荐用于抗体和蛋白质缀合的高比活性等级。
碱性磷酸酶可用于酪蛋白和其他蛋白质的去磷酸化。碱性磷酸酶还可用于 DNA 或 RNA 的 5′-末端的去磷酸化，以防止自我连接。经碱性磷酸酶的去磷酸化作用后，DNA 或 RNA 还可被放射性标记磷酸盐标记（通过 T4 多核苷酸激酶）。
生化/生理作用
该酶对醇类、胺类、焦磷酸盐以及酚类的磷酸酯具有广泛的特异性。常用于蛋白质和核酸的去磷酸化。
物理属性
牛肠碱性磷酸酶为二聚膜衍生糖蛋白。至少存在三种同工酶，每个单体通常拥有两个 N-连接多糖和一个或多个 O-连接多糖。2 该酶需要锌以及镁或钙二价离子才能显示活性。
牛肠道碱性磷酸酶是一种二聚化的膜衍生糖蛋白。其存在至少三种异构体，每个单体通常具有两个N-连接的和一个或多个O-连接的聚糖。
单位定义
在pH 9.8,37℃条件下，一个DEA单位可每分钟水解1 μmole的4-硝基苯基磷酸酯。（一个甘氨酸单位相当于~3个DEA单位）
外形
40%甘油溶液，含6 mM Tris、6 mM MgCl2和0.12 mM ZnCl2，pH值约为7.6
分析说明
包装规格取决于 DEA 单位
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves
法规信息
动植物源性产品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Wei Chen et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 33(4), 691-703 (2017-11-18)
CCAAT/enhancer-binding protein α (C/ebpα) is critical for osteoclastogenesis by regulating osteoclast (OC) lineage commitment and is also important for OC differentiation and function in vitro. However, the role of C/ebpα in postnatal skeletal development has not been reported owing to
Tuanmao Guo et al.
Journal of orthopaedic surgery and research, 16(1), 313-313 (2021-05-16)
Growing evidence has implicated core-binding factor beta (Cbfb) as a contributor to osteoblast differentiation, which plays a key role in fracture healing. Herein, we aimed to assess whether Cbfb affects osteoblast differentiation after fibula fracture. Initially, we established a Cbfb
Jun Tang et al.
The Journal of biological chemistry, 295(33), 11669-11681 (2020-06-24)
Despite years of research investigating osteoblast differentiation, the mechanisms by which transcription factors regulate osteoblast maturation, bone formation, and bone homeostasis is still unclear. It has been reported that runt-related transcription factor 1 (Runx1) is expressed in osteoblast progenitors, pre-osteoblasts
Sudha B Singh et al.
Scientific reports, 10(1), 3107-3107 (2020-02-23)
Intestinal alkaline phosphatase (IAP) regulates bicarbonate secretion, detoxifies lipopolysaccharide (LPS), regulates gut microbes, and dephosphorylates proinflammatory nucleotides. IAP also exhibits anti-inflammatory effects in a Toll-like Receptor-4 (TLR-4) dependent manner. However, it is not known whether IAP induces autophagy. We tested
A D Bakker et al.
Journal of dental research, 93(4), 394-399 (2014-02-05)
Mechanosensitive osteocytes regulate bone mass in adults. Interleukin 6 (IL-6), such as present during orthodontic tooth movement, also strongly affects bone mass, but little is known about the effect of IL-6 on osteocyte function. Therefore we aimed to determine in
实验方案
Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)
碱性磷酸酶的酶法测定，二乙醇胺法（EC 3.1.3.1)
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门