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应用
β-N-乙酰氨基葡萄糖苷酶是用于水解 N-乙酰基-β-D-氨基葡萄糖苷和 N-乙酰基-β-D-半乳糖胺的溶酶体酶。基于 β-GlcNAc 和 β-GalNAcc 的有效转糖基作用，它用于寡糖的化学酶促合成。其可能是研究阿尔茨海默病的有用工具 。来自洋刀豆的乙酰氨基葡萄糖苷酶已被用于研究生物膜的酶解离 。
生化/生理作用
这种酶，有时称为 β-N-乙酰氨基己糖苷酶，据报道可释放末端 β-连接来自多种底物的 N-乙酰葡糖胺和 N-乙酰半乳糖胺。β-N-酰基葡糖胺酶的活性可以用显色底物对硝基苯基-N-乙酰基-β-D-氨基葡萄糖苷测定。 β-N-酰基葡糖胺酶水解末端非还原性 N-乙酰基-D-己糖胺残基。这种酶含有两种主要的同工酶，Hex A（异源二聚体）和 Hex B（同源二聚体）。N-乙酰氨基葡萄糖、乙酰胺、N-2-乙酰胺基-2-脱氧葡萄糖胺、N-乙酰诺吉瑞霉素和 N-乙酰脱氧诺吉瑞霉素是已知的抑制剂。
这种酶，有时称为 β-N-乙酰氨基己糖苷酶，据报道可释放末端 β-连接来自多种底物的 N-乙酰葡糖胺和 N-乙酰半乳糖胺。
单位定义
一个单位将在 pH5.0、25 °C下每分钟将 1.0μmole 对硝基苯基 N-乙酰基-β-D-氨基葡萄糖水解成对硝基苯酚和N-乙酰基-D-葡糖胺。
外形
2.5 M 混悬液 (NH4)2SO4，pH 7.0
分析说明
在 pH 4.0 时，对硝基苯基 β-N-乙酰氨基半乳糖在 pH 5.0 时水解速率约为对硝基苯基 β-N-乙酰氨基葡萄糖水解速率的 50%。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物源性产品
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以硫酸铵悬浮液形式提供的酶的使用指南
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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