A1949
Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal
clone RG-96, purified from hybridoma cell culture
别名:
Monoclonal Anti-Rabbit IgG (γ-chain specific)
生物来源
mouse
质量水平
偶联物
peroxidase conjugate
抗体形式
purified immunoglobulin
抗体产品类型
secondary antibodies
克隆
RG-96, monoclonal
表单
lyophilized powder
种属反应性
rabbit
请勿与下列物质发生反应
goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken
包装
vial of 0.5 mL
技术
direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot: 1:160,000 using total cell extract of HeLa cells (5-10 μg/well)
同位素/亚型
IgG1
储存温度
2-8°C
靶向翻译后修饰
unmodified
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一般描述
Anti-Rabbit IgG (γ-chain specific)-Peroxidase antibody, Mouse monoclonal is a monoclonal anti-rabbit IgG conjugated to horseradish peroxidase. Monoclonal antirabbit IgG is derived from the RG-96 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rabbit IgG.
Rabbit IgGs are often used as primary antibodies in several immunological assays. Thus, polyclonal anti-rabbit IgGs are usually used as secondary antibodies in such applications. However, polyclonal antibodies to rabbit IgGs can also cross-react with IgGs from other species, especially with human IgGs, and thereby lead to a lack of specificity in test results. The use of monoclonal anti-rabbit IgG that is specific for only rabbit IgG improves the accuracy of immunological assays and thus, can be useful for various research applications.
The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.
免疫原
Rabbit IgG
应用
Monoclonal Anti-Rabbit IgG (γ-chain specific)- Peroxidase has been used in
- various immunochemical assays like enzyme linked immunosorbent assay (ELISA)
- westernblotting
- immunohistology
- dot immunobinding assay
外形
Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.
制备说明
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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警示用语：
Warning
危险声明
危险分类
Skin Sens. 1
储存分类代码
13 - Non Combustible Solids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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