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安全信息

A1543

Sigma-Aldrich

抗 人 IgG（全分子）-碱性磷酸酶 山羊抗

IgG fraction of antiserum, buffered aqueous solution

别名:

Goat Anti-Human IgG (whole molecule)−AP

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About This Item

MDL编号:
MFCD00162453
UNSPSC代码:
12352203
NACRES:
NA.46

生物来源

goat

质量水平

200

重组

expressed in goat

偶联物

alkaline phosphatase conjugate

抗体形式

IgG fraction of antiserum

抗体产品类型

secondary antibodies

克隆

polyclonal

表单

buffered aqueous solution

技术

direct ELISA: 1:20,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot (chemiluminescent): 1:30,000

运输

wet ice

储存温度

2-8°C

靶向翻译后修饰

unmodified

相关类别

一般描述

人IgG是糖蛋白抗体，含有两条相等的轻链和一对相同的重链，其分子量分别为 50kDa 和 25kDa。IgG具有四种不同亚型，范围从IgG1到IgG4。这些抗体可调节机体对过敏和病原性感染的免疫反应。IgG还与补体结合和自身免疫疾病有关。山羊抗人IgG与人IgG结合。免疫球蛋白G（IgG）属于免疫球蛋白家族，是一种广泛表达的血清抗体。该抗体的一级结构还包含连接两条重链、连接重链和轻链，并位于链内的二硫键。

免疫原

从人血清中纯化的 IgG

应用

人血清IgG水平通过固相ELISA测定，即使用碱性磷酸酶结合的山羊抗人IgG抗体，在37°C下培育1小时。该实验使用0.1%对硝基苯磷酸酯（Sigma）作为底物进行测定。通过蛋白质印迹分析，使用稀释比例为1:1000的碱性磷酸酶结合山羊抗人IgG抗体，检测人血清中对汉城病毒（80/39株）的抗体。采用氮蓝四唑和5-溴-4-氯-3-吲哚-磷酸酯底物（Sigma）进行印迹。
羊抗人 IgG 也可用于斑点印迹 (1：30000) 和免疫组化 (1：50) 应用。

外形

0.05 MTris (pH 8.0) 溶液，含 1% 牛血清白蛋白、1 mM MgCl 2 和 15 mM 叠氮化钠

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

含少量动物源组分生物产品
常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000341554
0000328714
0000329427
0000341554
0000328714

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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. What is the source of the alkaline phosphatase that is used to make Product A1543, Anti-Human IgG (whole molecule)-Alkaline Phosphatase antibody produced in goat?

    Product A1543, Anti-Human IgG (whole molecule) -Alkaline Phosphatase antibody produced in goat, is prepared using alkaline phosphatase from bovine intestinal mucosa.

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

C T Ravirajan et al.
Rheumatology (Oxford, England), 43(4), 442-447 (2004-03-17)
There is considerable evidence suggesting that anti-double-stranded deoxyribonucleic acid (anti-dsDNA) antibodies are involved in the pathogenesis of lupus nephritis. It was shown previously using severe combined immune deficient (SCID) mice that when the hybridomas secreting human immunoglobulin G (IgG) anti-dsDNA
Jens Bukh et al.
Journal of medical virology, 80(1), 87-94 (2007-11-28)
GB virus-B (GBV-B) is the virus most closely related to hepatitis C virus (HCV). Thus, we have used GBV-B infection of tamarins, which develop acute hepatitis following experimental infection, as a surrogate model to study protective immunity. As challenge virus
Molouk Beiromvand et al.
Journal of infection in developing countries, 13(2), 154-161 (2020-02-10)
Cystic Echinococcosis (CE) and toxocariasis caused by the larval stages of intestinal dog worms including Echinococcus granulosus and Toxocara spp. are among the most widespread zoonotic diseases. Four hundred municipal waste collectors were serologically evaluated for CE and toxocariasis. To
L Lledó et al.
Journal of medical microbiology, 51(10), 861-865 (2002-11-19)
Data relating to hantavirus infection in Spain are scarce and limited to rural areas. The aim of this work was to study the seroprevalence of hantavirus infection in the Autonomous Community of Madrid (ACM), a region containing both rural and
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We established the first prospective cohort to understand how infection with dengue virus is influenced by vector-specific determinants such as humoral immunity to Aedes aegypti salivary proteins. Children aged 2-9 years were enrolled in the PAGODAS (Pediatric Assessment Group of
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