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Merck
CN

A1418

抗 小鼠 IgG(Fc 特异性)-碱性磷酸酶 山羊抗

affinity isolated antibody, buffered aqueous solution

别名:

Goat Anti-Mouse IgG (Fc specific)–AP

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
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产品名称

抗 小鼠 IgG(Fc 特异性)-碱性磷酸酶 山羊抗, affinity isolated antibody, buffered aqueous solution

biological source

goat

recombinant

expressed in goat

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

should not react with

human

technique(s)

direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25
western blot: 1:50,000-1:100,000 using total cell extract of HeLa cells

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

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Application

碱性磷酸酶缀合的山羊抗小鼠 Fc 特异性抗体在 ELISA 测定中用作二抗,在 PBS/0.1% Tween 和 1% BSA中以 1:1000 的稀释度,在 37°C 下持续 1.5 小时。该抗体经开发使用磷酸 4-硝基苯酯(Sigma)作为底物,在 37°C 下,持续 30分钟。
抗小鼠 IgG(Fc 特异性)-碱性磷酸酶抗体可用于 ELISPOT(免疫斑点)测定。它也可用于免疫组化和蛋白质印迹。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

IgG 抗体在补体激活、吞噬作用、胎盘转运和细胞表面受体结合等体液免疫应答中起关键作用。抗小鼠 IgG(Fc 特异性)-碱性磷酸酶抗体(以1:10,000 的稀释度采用PBS-Tween 稀释)可用于 ELISA。它也可用于免疫印迹。山羊抗小鼠 IgG(Fc 特异性)-碱性磷酸酶抗体与小鼠 IgG Fc 片段、IgG 及其亚类 IgG1、IgG2a、IgG2b 和 IgG3 发生特异性反应,但不与人 IgG 和小鼠 IgA 或 IgM 发生反应。

Immunogen

纯化的小鼠 IgG Fc 片段。

Other Notes

用人IgG吸附抗体。

Physical form

0.05 MTris (pH 8.0) 溶液,含 1% 牛血清白蛋白、1 mM MgCl 2 和 15 mM 叠氮化钠

Preparation Note

经过吸附以减少人体样本的背景染色。

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存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
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分析证书(COA)

Lot/Batch Number

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Ajeet Singh Yadav et al.
Biomicrofluidics, 17(5), 054101-054101 (2023-09-18)
Effective immunotherapies activate natural antitumor immune responses in patients undergoing treatment. The ability to monitor immune activation in response to immunotherapy is critical in measuring treatment efficacy over time and across patient cohorts. Protein arrays are systematically arranged, large collections
V L Motin et al.
Infection and immunity, 62(10), 4192-4201 (1994-10-01)
LcrV (V antigen), a known unstable 37.3-kDa monomeric peptide encoded on the ca. 70-kb Lcr plasmid of Yersinia pestis, Yersinia pseudotuberculosis, and Yersinia enterocolitica, has been implicated as a regulator of the low-calcium response, virulence factor, and protective antigen. In
D J Lewis et al.
European journal of immunology, 21(9), 2087-2094 (1991-09-01)
The immune response to cholera toxin B subunit given orally was studied in 13 human volunteers. A serum IgG and IgA antitoxin response was observed, which was boosted by a second immunization. Using an immunospot assay, cells spontaneously secreting anti-toxin
N Miquel et al.
Parasite immunology, 27(3), 79-88 (2005-05-11)
Pigs single inoculated with Ascaris suum eggs expel the majority of larvae between days 14 and 21 post inoculation (p.i.), but the role of the immune system in expulsion is unclear. To investigate the dynamics of immune responses before, during
Development of a Surface Plasmon Resonance?Based
Immunoassay for Listeria monocytogenes
Leonard P, et al.
Journal of Food Protection, 68(4), 728-735 null

商品

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

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