生物来源
goat
质量水平
重组
expressed in goat
偶联物
alkaline phosphatase conjugate
抗体形式
affinity isolated antibody
抗体产品类型
secondary antibodies
克隆
polyclonal
形式
buffered aqueous solution
种属反应性
mouse
请勿与下列物质发生反应
human
技术
direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25
western blot: 1:50,000-1:100,000 using total cell extract of HeLa cells
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
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相关类别
一般描述
IgG 抗体在补体激活、吞噬作用、胎盘转运和细胞表面受体结合等体液免疫应答中起关键作用。抗小鼠 IgG（Fc 特异性）-碱性磷酸酶抗体（以1：10,000 的稀释度采用PBS-Tween 稀释）可用于 ELISA。它也可用于免疫印迹。山羊抗小鼠 IgG（Fc 特异性）-碱性磷酸酶抗体与小鼠 IgG Fc 片段、IgG 及其亚类 IgG1、IgG2a、IgG2b 和 IgG3 发生特异性反应，但不与人 IgG 和小鼠 IgA 或 IgM 发生反应。
免疫原
纯化的小鼠 IgG Fc 片段。
应用
抗小鼠 IgG（Fc 特异性）-碱性磷酸酶抗体可用于 ELISPOT（免疫斑点）测定。它也可用于免疫组化和蛋白质印迹。
碱性磷酸酶缀合的山羊抗小鼠 Fc 特异性抗体在 ELISA 测定中用作二抗，在 PBS/0.1% Tween 和 1% BSA中以 1:1000 的稀释度，在 37°C 下持续 1.5 小时。该抗体经开发使用磷酸 4-硝基苯酯（Sigma）作为底物，在 37°C 下，持续 30分钟。
其他说明
用人IgG吸附抗体。
外形
0.05 MTris (pH 8.0) 溶液，含 1% 牛血清白蛋白、1 mM MgCl 2 和 15 mM 叠氮化钠
制备说明
经过吸附以减少人体样本的背景染色。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
含少量动物源组分生物产品
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Effective immunotherapies activate natural antitumor immune responses in patients undergoing treatment. The ability to monitor immune activation in response to immunotherapy is critical in measuring treatment efficacy over time and across patient cohorts. Protein arrays are systematically arranged, large collections
N Miquel et al.
Parasite immunology, 27(3), 79-88 (2005-05-11)
Pigs single inoculated with Ascaris suum eggs expel the majority of larvae between days 14 and 21 post inoculation (p.i.), but the role of the immune system in expulsion is unclear. To investigate the dynamics of immune responses before, during
D J Lewis et al.
European journal of immunology, 21(9), 2087-2094 (1991-09-01)
The immune response to cholera toxin B subunit given orally was studied in 13 human volunteers. A serum IgG and IgA antitoxin response was observed, which was boosted by a second immunization. Using an immunospot assay, cells spontaneously secreting anti-toxin
V L Motin et al.
Infection and immunity, 62(10), 4192-4201 (1994-10-01)
LcrV (V antigen), a known unstable 37.3-kDa monomeric peptide encoded on the ca. 70-kb Lcr plasmid of Yersinia pestis, Yersinia pseudotuberculosis, and Yersinia enterocolitica, has been implicated as a regulator of the low-calcium response, virulence factor, and protective antigen. In
Marta Baranowska et al.
Vaccine, 33(49), 6988-6996 (2015-09-22)
Vaccination is at present the most efficient way of preventing influenza infections. Currently used inactivated influenza vaccines can induce virus-neutralizing antibodies that are protective against a particular influenza strain, but hamper the induction of cross-protective T-cell responses to later infections.
商品
ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.
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