描述
zwitterionic
质量水平
检测方案
≥95.0% (HPLC)
形式
powder
分子量
average mol wt >10,000
聚集数
≥23
储存温度
2-8°C
InChI
1S/C22H46N2O4S/c1-4-5-6-7-8-9-10-11-12-13-14-17-22(25)23-18-15-19-24(2,3)20-16-21-29(26,27)28/h4-21H2,1-3H3,(H-,23,25,26,27,28)
InChI key
UTSXERRKRAEDOV-UHFFFAOYSA-N
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一般描述
ASB-14包含尾部烷基基团和头部氨基磺基甜菜碱基团。
应用
ASB-14 用作再水合缓冲液成分，用于制备来自杜氏利什曼原虫（ Leishmania donovani）的蛋白组学样品。
两性离子洗涤剂。用于 2D 电泳时溶解蛋白质（包括膜蛋白）。
生化/生理作用
ASB-14可用于蛋白酶体提取，特别适用膜相关蛋白。与CHAPS（3-（3-胆酰胺基丙基）二甲基铵基）-1-丙烷磺酸盐一起，用于蛋白增溶，即使在硫脲存在的情况下，也可以起作用。
分析说明
ASB-14（脒基磺基甜菜碱-14）是一种膜增溶两性离子洗涤剂（中链脒基磺基甜菜碱表面活性剂），可用于蛋白质和大分子研究，用于蛋白质的制备提取和增溶，以通过 2D-电泳等方法进行分离和分析。ASB-14 有望在组织工程中用于异种支架材料的去抗原 (AR) 制备。ASB-14 可用于开发和研究两性离子胶束。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
Nityakalyani Srinivas et al.
Science (New York, N.Y.), 327(5968), 1010-1013 (2010-02-20)
Antibiotics with new mechanisms of action are urgently required to combat the growing health threat posed by resistant pathogenic microorganisms. We synthesized a family of peptidomimetic antibiotics based on the antimicrobial peptide protegrin I. Several rounds of optimization gave a
Francisella t ularensis Proteome: Low Levels of ASB-14 Facilitate the Visualization of Membrane Proteins in Total Protein Extracts
Twine SM, et al.
Journal of Proteome Research, 4(5), 1848-1854 (2005)
Maelene L Wong et al.
Acta biomaterialia, 9(5), 6492-6501 (2013-01-17)
The ability of residual antigens on decellularized tissue to elicit the immune response upon implantation motivates development of a more rigorous antigen removal (AR) process for xenogeneic scaffold generation. Antigen removal strategies promoting solubilization of hydrophilic proteins (predominantly cytoplasmic) enhance
Zhiquan Chen et al.
Free radical biology & medicine, 102, 77-86 (2016-11-15)
Nrf2/ARE signaling pathway is a crucial cellular defense system to cope with oxidative stress, which is adaptively activated, in diabetic condition that is not efficient enough to resist the oxidative stress provoked by hyperglycemia. We have previously demonstrated that Connexin43
Daniel Martins-de-Souza et al.
Briefings in functional genomics & proteomics, 6(1), 70-75 (2007-06-09)
Protein extraction is the most important step to reveal a proteome by Two-Dimensional Gel Electrophoresis. Usually, the urea/thiourea based standard protein extraction buffer (SB) is combined with detergents with the aim of achieving better resolution and solubilization of different classes
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