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文件

A1287

Sigma-Aldrich

5-阿扎胞苷

Hybri-Max™, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

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别名:
4-氨基-1-(β-D-呋喃核糖基)-1,3,5-三嗪-2(1H)-酮, 阿扎胞苷
经验公式（希尔记法）:
C8H12N4O5
CAS号:
320-67-2
分子量:
244.20
Beilstein:
620461
EC 号:
206-280-2
MDL编号:
MFCD00006539
UNSPSC代码:
12352207
PubChem化学物质编号:
24890525
NACRES:
NA.75

生物来源

synthetic (organic)

质量水平

200

等级

Hybri-Max™

无菌性

γ-irradiated

产品线

BioXtra

形式

lyophilized powder

技术

cell culture | hybridoma: suitable

杂质

endotoxin, tested

mp

226-232 °C (dec.) (lit.)

溶解性

acetic acid: water (1:1): 5 mg/mL, clear, colorless

抗生素抗菌谱

viruses

作用机制

DNA synthesis | interferes

储存温度

−20°C

SMILES字符串

NC1=NC(=O)N(C=N1)[C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O

InChI

1S/C8H12N4O5/c9-7-10-2-12(8(16)11-7)6-5(15)4(14)3(1-13)17-6/h2-6,13-15H,1H2,(H2,9,11,16)/t3-,4-,5-,6-/m1/s1

InChI key

NMUSYJAQQFHJEW-KVTDHHQDSA-N

基因信息

human ... DNMT1(1786) , DNMT3A(1788)

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相关类别

一般描述

化学结构：核苷

应用

5-氮杂胞苷可用于诱导成人骨髓间充质干细胞的体外心肌向分化。它也可用于研究5-氮杂胞苷对骨髓来源的免疫调节性抑制细胞（MDSC）和调节性T细胞（Treg）的影响。
用于研究DNA掺入分子对DNA甲基化过程的影响。

生化/生理作用

5-氮杂胞苷是脱氧胞苷类似物和去甲基化剂。它可作为急性骨髓性白血病的潜在抗肿瘤药。5-氮杂胞苷通过抑制DNA甲基化来激活被抑制的基因。5-氮杂胞苷还通过改变RNA功能和稳定性来影响蛋白质合成。
一种强效生长抑制剂及细胞毒素剂，抑制 DNA 甲基转移酶，一种基因表达、基因激活和沉默的重要调节机制。

重悬

10mL 无菌细胞培养基可复原一小瓶本品。储备液足以制备 500mL 培养基。最终工作浓度：10μM 5-氮杂胞嘧啶核苷。

法律信息

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Chronic 1 - Carc. 1A - Muta. 2 - Repr. 1B - STOT RE 1

WGK

WGK 3

个人防护装备

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the concentration of Product A1287, 5-Azacytidine - Hybri-Max™?

    The working concentration is 10 μM, so the 50X stock is 500 μM.

  4. How much is contained in the 1 vial of Product A1287, 5-Azacytidine - Hybri-Max™?

    1 vial contains 1.2 mg.

  5. How is Product A1287, 5-Azacytidine - Hybri-Max™, to be dissolved? And once dissolved, is the solution stable?

    Dissolve each 1.2 mg vial in 10 mL of culture medium. This compound is very unstable in aqueous solutions; we have found a 10% loss after just 2-3 hours at room temperature in phosphate buffer pH 7.4, even faster loss at 50°C. We generally recommend keeping solutions on ice during the course of a day. If stock solution must be kept, store them in the freezer at -20°C in single use aliquots.  

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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