跳转至内容
Merck
CN

A1287

5-阿扎胞苷

Hybri-Max, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

别名:

4-氨基-1-(β-D-呋喃核糖基)-1,3,5-三嗪-2(1H)-酮, 阿扎胞苷

登录 查看组织和合同定价。

选择尺寸


关于此项目

经验公式(希尔记法):
C8H12N4O5
化学文摘社编号:
分子量:
244.20
MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
EC Number:
206-280-2
Beilstein/REAXYS Number:
620461
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

产品名称

5-阿扎胞苷, Hybri-Max, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

InChI key

NMUSYJAQQFHJEW-KVTDHHQDSA-N

InChI

1S/C8H12N4O5/c9-7-10-2-12(8(16)11-7)6-5(15)4(14)3(1-13)17-6/h2-6,13-15H,1H2,(H2,9,11,16)/t3-,4-,5-,6-/m1/s1

SMILES string

NC1=NC(=O)N(C=N1)[C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O

biological source

synthetic (organic)

grade

Hybri-Max

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

mp

226-232 °C (dec.) (lit.)

solubility

acetic acid: water (1:1): 5 mg/mL, clear, colorless

antibiotic activity spectrum

viruses

mode of action

DNA synthesis | interferes

storage temp.

−20°C

Quality Level

Gene Information

正在寻找类似产品? 访问 产品对比指南

Application

5-氮杂胞苷可用于诱导成人骨髓间充质干细胞的体外心肌向分化。它也可用于研究5-氮杂胞苷对骨髓来源的免疫调节性抑制细胞(MDSC)和调节性T细胞(Treg)的影响。
用于研究DNA掺入分子对DNA甲基化过程的影响。

Biochem/physiol Actions

5-氮杂胞苷是脱氧胞苷类似物和去甲基化剂。它可作为急性骨髓性白血病的潜在抗肿瘤药。5-氮杂胞苷通过抑制DNA甲基化来激活被抑制的基因。5-氮杂胞苷还通过改变RNA功能和稳定性来影响蛋白质合成。
一种强效生长抑制剂及细胞毒素剂,抑制 DNA 甲基转移酶,一种基因表达、基因激活和沉默的重要调节机制。
强效生长抑制剂及细胞毒素剂,抑制 DNA 甲基转移酶。

General description

化学结构:核苷

Preparation Note

10mL 无菌细胞培养基可复原一小瓶本品。储备液足以制备 500mL 培养基。最终工作浓度:10μM 5-氮杂胞嘧啶核苷。

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 1 - Carc. 1A - Muta. 2 - Repr. 1B - STOT RE 1

存储类别

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the concentration of Product A1287, 5-Azacytidine - Hybri-Max?

    The working concentration is 10 μM, so the 50X stock is 500 μM.

  4. How much is contained in the 1 vial of Product A1287, 5-Azacytidine - Hybri-Max?

    1 vial contains 1.2 mg.

  5. How is Product A1287, 5-Azacytidine - Hybri-Max, to be dissolved? And once dissolved, is the solution stable?

    Dissolve each 1.2 mg vial in 10 mL of culture medium. This compound is very unstable in aqueous solutions; we have found a 10% loss after just 2-3 hours at room temperature in phosphate buffer pH 7.4, even faster loss at 50°C. We generally recommend keeping solutions on ice during the course of a day. If stock solution must be kept, store them in the freezer at -20°C in single use aliquots.  

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

In vitro cardiomyogenic differentiation of adult human bone marrow mesenchymal stem cells. The role of 5-azacytidine
Antonitsis P, et al.
Interactive Cardiovascular and Thoracic Surgery, 6(5), 593-597 (2007)
5-Azacytidine treatment sensitizes tumor cells to T-cell mediated cytotoxicity and modulates NK cells in patients with myeloid malignancies
Gang AO, et al.
Blood Cancer Journal, 4(3), e197-e197 (2014)
S Massah et al.
BMC genetics, 21(1), 3-3 (2020-01-17)
DNA methylation is an epigenetic modification that mainly repress expression of genes essential during embryogenesis and development. There are key ATPase-dependent enzymes that read or write DNA methylation to remodel chromatin and regulate gene expression. Structural maintenance of chromosome hinge
5-azacytidine treatment reorganizes genomic histone modification patterns
Komashko VM and Farnham PJ
Epigenetics, 5(3), 229-240 (2010)
DNA methyltransferase inhibitors for cancer therapy
Epigenetics, 5(3), 265-290 (2015)

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持