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Merck
CN

A0412

抗-小鼠多价免疫球蛋白(G,A,M)−过氧化物酶 山羊抗

affinity isolated antibody

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA (d)
Citations:
34
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biological source

goat

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

mouse

technique(s)

direct ELISA: 1:10,000 using IgG, IgA, IgM

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

General description

免疫球蛋白是B细胞在应对抗原时产生的蛋白质,用于调节对细菌和病毒的免疫应答。IgG还可用于调节补体结合和胎盘传输。IgA在黏膜免疫中起着至关重要的作用,因为其限制了病原体进入黏膜。IgM是最大的抗体,具有五聚体结构,可调节多重亲和性并清除凋亡细胞。山羊抗小鼠多价免疫球蛋白(G,A,M)-过氧化物酶抗体与小鼠IgG、IgA和IgM结合。

Application

使用过氧化物酶偶联的抗小鼠多价免疫球蛋白抗体作为二抗,通过ELISA检测弓形虫感染小鼠血清中的抗体。 使用过氧化物酶偶联的山羊抗小鼠多价抗体(按1:10,000稀释于PBST中)并在21-23°C下孵育1小时,通过抑制ELISA法测定特定单克隆抗体的相对结合亲和力。

Physical form

0.01 M磷酸盐缓冲液(pH 7.4,含有带保存剂的1%牛血清白蛋白)溶液。

Preparation Note

按照Wilson, M.B.,和Nakane, P.K.在由阿姆斯特丹Elsevier/北荷兰生物医学出版社出版的免疫荧光及相关染色技术(1978)一书中所描述的高碘酸盐法而制备。

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除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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signalword

Warning

hcodes

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
低风险生物材料
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Nicole Forster et al.
Journal of immunology (Baltimore, Md. : 1950), 178(11), 6941-6948 (2007-05-22)
Conditional knock-in mice expressing a histone acetyltransferase-deficient version of the transcriptional coregulator p300 exclusively in B lymphocytes die prematurely with full penetrance. The mice develop an autoimmune disease similar to systemic lupus erythematosus in its pathological manifestations, such as splenomegaly
M Chen et al.
Infection and immunity, 68(9), 4893-4899 (2000-08-19)
Formation of anti-Toxoplasma gondii HSP70 (TgHSP70) antibody cross-reactive to mouse HSP70 (mHSP70) was observed in the sera of BALB/c (a resistant strain) and C57BL/6 (B6; a susceptible strain) mice after peroral infection with T. gondii cysts of the Fukaya strain.
Hong Xin et al.
Infection and immunity, 74(7), 4310-4321 (2006-06-23)
We previously reported the enhanced resistance of monoclonal antibodies B6.1 (an immunoglobulin M [IgM]) and C3.1 (an IgG3) against experimental candidiasis. Both MAbs recognize the same fungal epitope. We have since found that a highly passaged B6.1 hybridoma (hp-B6.1) resulted
Willem-Jan M Schellekens et al.
Anesthesiology, 117(2), 329-338 (2012-06-23)
Mechanical ventilation induces diaphragm muscle atrophy, which plays a key role in difficult weaning from mechanical ventilation. The signaling pathways involved in ventilator-induced diaphragm atrophy are poorly understood. The current study investigated the role of Toll-like receptor 4 signaling in
Genna Davies et al.
Journal of fungi (Basel, Switzerland), 7(1) (2021-01-06)
Invasive pulmonary aspergillosis (IPA) caused by Aspergillus fumigatus is a life-threatening lung disease of immunocompromised patients. Diagnosis currently relies on non-specific chest CT, culture of the fungus from invasive lung biopsy, and detection of the cell wall carbohydrate galactomannan (GM)

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